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Generation of a Gluconobacter oxydans knockout collection for improved extraction of rare earth elements

Author

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  • Alexa M. Schmitz

    (Cornell University)

  • Brooke Pian

    (Cornell University)

  • Sean Medin

    (Cornell University)

  • Matthew C. Reid

    (Cornell University)

  • Mingming Wu

    (Cornell University)

  • Esteban Gazel

    (Cornell University)

  • Buz Barstow

    (Cornell University)

Abstract

Bioleaching of rare earth elements (REEs), using microorganisms such as Gluconobacter oxydans, offers a sustainable alternative to environmentally harmful thermochemical extraction, but is currently not very efficient. Here, we generate a whole-genome knockout collection of single-gene transposon disruption mutants for G. oxydans B58, to identify genes affecting the efficacy of REE bioleaching. We find 304 genes whose disruption alters the production of acidic biolixiviant. Disruption of genes underlying synthesis of the cofactor pyrroloquinoline quinone (PQQ) and the PQQ-dependent membrane-bound glucose dehydrogenase nearly eliminates bioleaching. Disruption of phosphate-specific transport system genes enhances bioleaching by up to 18%. Our results provide a comprehensive roadmap for engineering the genome of G. oxydans to further increase its bioleaching efficiency.

Suggested Citation

  • Alexa M. Schmitz & Brooke Pian & Sean Medin & Matthew C. Reid & Mingming Wu & Esteban Gazel & Buz Barstow, 2021. "Generation of a Gluconobacter oxydans knockout collection for improved extraction of rare earth elements," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27047-4
    DOI: 10.1038/s41467-021-27047-4
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    References listed on IDEAS

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    1. Michael Baym & Lev Shaket & Isao A. Anzai & Oluwakemi Adesina & Buz Barstow, 2016. "Rapid construction of a whole-genome transposon insertion collection for Shewanella oneidensis by Knockout Sudoku," Nature Communications, Nature, vol. 7(1), pages 1-13, December.
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