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Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution

Author

Listed:
  • Gareth O. S. Williams

    (University of Edinburgh)

  • Elvira Williams

    (Durham University)

  • Neil Finlayson

    (University of Edinburgh, King’s Buildings)

  • Ahmet T. Erdogan

    (University of Edinburgh, King’s Buildings)

  • Qiang Wang

    (University of Edinburgh)

  • Susan Fernandes

    (University of Edinburgh)

  • Ahsan R. Akram

    (University of Edinburgh)

  • Kev Dhaliwal

    (University of Edinburgh)

  • Robert K. Henderson

    (University of Edinburgh, King’s Buildings)

  • John M. Girkin

    (Durham University)

  • Mark Bradley

    (University of Edinburgh)

Abstract

The use of optical techniques to interrogate wide ranging samples from semiconductors to biological tissue for rapid analysis and diagnostics has gained wide adoption over the past decades. The desire to collect ever more spatially, spectrally and temporally detailed optical signatures for sample characterization has specifically driven a sharp rise in new optical microscopy technologies. Here we present a high-speed optical scanning microscope capable of capturing time resolved images across 512 spectral and 32 time channels in a single acquisition with the potential for ~0.2 frames per second (256 × 256 image pixels). Each pixel in the resulting images contains a detailed data cube for the study of diverse time resolved light driven phenomena. This is enabled by integration of system control electronics and on-chip processing which overcomes the challenges presented by high data volume and low imaging speed, often bottlenecks in previous systems.

Suggested Citation

  • Gareth O. S. Williams & Elvira Williams & Neil Finlayson & Ahmet T. Erdogan & Qiang Wang & Susan Fernandes & Ahsan R. Akram & Kev Dhaliwal & Robert K. Henderson & John M. Girkin & Mark Bradley, 2021. "Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26837-0
    DOI: 10.1038/s41467-021-26837-0
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    References listed on IDEAS

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    1. Wiebke Jahr & Benjamin Schmid & Christopher Schmied & Florian O. Fahrbach & Jan Huisken, 2015. "Hyperspectral light sheet microscopy," Nature Communications, Nature, vol. 6(1), pages 1-7, November.
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