IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-25691-4.html
   My bibliography  Save this article

Designing efficient genetic code expansion in Bacillus subtilis to gain biological insights

Author

Listed:
  • Devon A. Stork

    (Harvard Medical School
    Harvard University)

  • Georgia R. Squyres

    (Harvard University)

  • Erkin Kuru

    (Harvard Medical School
    Wyss Institute for Biologically Inspired Engineering)

  • Katarzyna A. Gromek

    (University of Wisconsin-Madison)

  • Jonathan Rittichier

    (Harvard Medical School
    Wyss Institute for Biologically Inspired Engineering)

  • Aditya Jog

    (Harvard Medical School)

  • Briana M. Burton

    (University of Wisconsin-Madison)

  • George M. Church

    (Harvard Medical School
    Wyss Institute for Biologically Inspired Engineering)

  • Ethan C. Garner

    (Harvard University)

  • Aditya M. Kunjapur

    (Harvard Medical School
    University of Delaware)

Abstract

Bacillus subtilis is a model gram-positive bacterium, commonly used to explore questions across bacterial cell biology and for industrial uses. To enable greater understanding and control of proteins in B. subtilis, here we report broad and efficient genetic code expansion in B. subtilis by incorporating 20 distinct non-standard amino acids within proteins using 3 different families of genetic code expansion systems and two choices of codons. We use these systems to achieve click-labelling, photo-crosslinking, and translational titration. These tools allow us to demonstrate differences between E. coli and B. subtilis stop codon suppression, validate a predicted protein-protein binding interface, and begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo. We expect that the establishment of this simple and easily accessible chemical biology system in B. subtilis will help uncover an abundance of biological insights and aid genetic code expansion in other organisms.

Suggested Citation

  • Devon A. Stork & Georgia R. Squyres & Erkin Kuru & Katarzyna A. Gromek & Jonathan Rittichier & Aditya Jog & Briana M. Burton & George M. Church & Ethan C. Garner & Aditya M. Kunjapur, 2021. "Designing efficient genetic code expansion in Bacillus subtilis to gain biological insights," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25691-4
    DOI: 10.1038/s41467-021-25691-4
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-25691-4
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-25691-4?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25691-4. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.