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Toward scalable biocatalytic conversion of 5-hydroxymethylfurfural by galactose oxidase using coordinated reaction and enzyme engineering

Author

Listed:
  • William R. Birmingham

    (The University of Manchester, Manchester Institute of Biotechnology)

  • Asbjørn Toftgaard Pedersen

    (Technical University of Denmark
    Novozymes A/S, Krogshoejvej 36)

  • Mafalda Dias Gomes

    (Technical University of Denmark)

  • Mathias Bøje Madsen

    (Technical University of Denmark)

  • Michael Breuer

    (BASF SE, White Biotechnology Research)

  • John M. Woodley

    (Technical University of Denmark)

  • Nicholas J. Turner

    (The University of Manchester, Manchester Institute of Biotechnology)

Abstract

5-Hydroxymethylfurfural (HMF) has emerged as a crucial bio-based chemical building block in the drive towards developing materials from renewable resources, due to its direct preparation from sugars and its readily diversifiable scaffold. A key obstacle in transitioning to bio-based plastic production lies in meeting the necessary industrial production efficiency, particularly in the cost-effective conversion of HMF to valuable intermediates. Toward addressing the challenge of developing scalable technology for oxidizing crude HMF to more valuable chemicals, here we report coordinated reaction and enzyme engineering to provide a galactose oxidase (GOase) variant with remarkably high activity toward HMF, improved O2 binding and excellent productivity (>1,000,000 TTN). The biocatalyst and reaction conditions presented here for GOase catalysed selective oxidation of HMF to 2,5-diformylfuran offers a productive blueprint for further development, giving hope for the creation of a biocatalytic route to scalable production of furan-based chemical building blocks from sustainable feedstocks.

Suggested Citation

  • William R. Birmingham & Asbjørn Toftgaard Pedersen & Mafalda Dias Gomes & Mathias Bøje Madsen & Michael Breuer & John M. Woodley & Nicholas J. Turner, 2021. "Toward scalable biocatalytic conversion of 5-hydroxymethylfurfural by galactose oxidase using coordinated reaction and enzyme engineering," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
  • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25034-3
    DOI: 10.1038/s41467-021-25034-3
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    Cited by:

    1. Martin Pfeiffer & Andrej Ribar & Bernd Nidetzky, 2023. "A selective and atom-economic rearrangement of uridine by cascade biocatalysis for production of pseudouridine," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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