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Switching at the ribosome: riboswitches need rProteins as modulators to regulate translation

Author

Listed:
  • Vanessa de Jesus

    (Johann Wolfgang Goethe-University Frankfurt)

  • Nusrat S. Qureshi

    (Johann Wolfgang Goethe-University Frankfurt)

  • Sven Warhaut

    (Johann Wolfgang Goethe-University Frankfurt)

  • Jasleen K. Bains

    (Johann Wolfgang Goethe-University Frankfurt)

  • Marina S. Dietz

    (Johann Wolfgang Goethe-University Frankfurt)

  • Mike Heilemann

    (Johann Wolfgang Goethe-University Frankfurt)

  • Harald Schwalbe

    (Johann Wolfgang Goethe-University Frankfurt)

  • Boris Fürtig

    (Johann Wolfgang Goethe-University Frankfurt)

Abstract

Translational riboswitches are cis-acting RNA regulators that modulate the expression of genes during translation initiation. Their mechanism is considered as an RNA-only gene-regulatory system inducing a ligand-dependent shift of the population of functional ON- and OFF-states. The interaction of riboswitches with the translation machinery remained unexplored. For the adenine-sensing riboswitch from Vibrio vulnificus we show that ligand binding alone is not sufficient for switching to a translational ON-state but the interaction of the riboswitch with the 30S ribosome is indispensable. Only the synergy of binding of adenine and of 30S ribosome, in particular protein rS1, induces complete opening of the translation initiation region. Our investigation thus unravels the intricate dynamic network involving RNA regulator, ligand inducer and ribosome protein modulator during translation initiation.

Suggested Citation

  • Vanessa de Jesus & Nusrat S. Qureshi & Sven Warhaut & Jasleen K. Bains & Marina S. Dietz & Mike Heilemann & Harald Schwalbe & Boris Fürtig, 2021. "Switching at the ribosome: riboswitches need rProteins as modulators to regulate translation," Nature Communications, Nature, vol. 12(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25024-5
    DOI: 10.1038/s41467-021-25024-5
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