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TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation

Author

Listed:
  • Klára Klimešová

    (Institute of Molecular Genetics, Czech Academy of Sciences)

  • Jitka Vojáčková

    (Institute of Molecular Genetics, Czech Academy of Sciences)

  • Nenad Radivojević

    (Institute of Molecular Genetics, Czech Academy of Sciences)

  • Franck Vandermoere

    (Institut de Génomique Fonctionnelle, University of Montpellier, CNRS, INSERM)

  • Edouard Bertrand

    (Institut de Génétique Moléculaire de Montpellier, University of Montpellier, CNRS
    Equipe labélisée Ligue Nationale Contre le Cancer
    Institut de Génétique Humaine)

  • Celine Verheggen

    (Institut de Génétique Moléculaire de Montpellier, University of Montpellier, CNRS
    Equipe labélisée Ligue Nationale Contre le Cancer
    Institut de Génétique Humaine)

  • David Staněk

    (Institute of Molecular Genetics, Czech Academy of Sciences)

Abstract

U5 snRNP is a complex particle essential for RNA splicing. U5 snRNPs undergo intricate biogenesis that ensures that only a fully mature particle assembles into a splicing competent U4/U6•U5 tri-snRNP and enters the splicing reaction. During splicing, U5 snRNP is substantially rearranged and leaves as a U5/PRPF19 post-splicing particle, which requires re-generation before the next round of splicing. Here, we show that a previously uncharacterized protein TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation. We provide evidence that TSSC4 associates with U5 snRNP chaperones, U5 snRNP and the U5/PRPF19 particle. Specifically, TSSC4 interacts with U5-specific proteins PRPF8, EFTUD2 and SNRNP200. We also identified TSSC4 domains critical for the interaction with U5 snRNP and the PRPF19 complex, as well as for TSSC4 function in tri-snRNP assembly. TSSC4 emerges as a specific chaperone that acts in U5 snRNP de novo biogenesis as well as post-splicing recycling.

Suggested Citation

  • Klára Klimešová & Jitka Vojáčková & Nenad Radivojević & Franck Vandermoere & Edouard Bertrand & Celine Verheggen & David Staněk, 2021. "TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23934-y
    DOI: 10.1038/s41467-021-23934-y
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