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Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs

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Listed:
  • Majid Haddad Momeni

    (Technical University of Denmark
    INRAE, Aix Marseille Univ, Biodiversité et Biotechnologie Fongiques (BBF))

  • Folmer Fredslund

    (The Novo Nordisk Center for Biosustainability)

  • Bastien Bissaro

    (INRAE, Aix Marseille Univ, Biodiversité et Biotechnologie Fongiques (BBF))

  • Olanrewaju Raji

    (University of Toronto)

  • Thu V. Vuong

    (University of Toronto)

  • Sebastian Meier

    (Technical University of Denmark)

  • Tine Sofie Nielsen

    (Technical University of Denmark)

  • Vincent Lombard

    (Architecture et Fonction des Macromolécules Biologiques, UMR 7257 CNRS, USC 1408, Aix Marseille Univ)

  • Bruno Guigliarelli

    (Aix-Marseille Univ, CNRS, UMR7281 Unité de Bioénergétique et Ingénierie des Protéines (BIP))

  • Frédéric Biaso

    (Aix-Marseille Univ, CNRS, UMR7281 Unité de Bioénergétique et Ingénierie des Protéines (BIP))

  • Mireille Haon

    (INRAE, Aix Marseille Univ, Biodiversité et Biotechnologie Fongiques (BBF))

  • Sacha Grisel

    (INRAE, Aix Marseille Univ, Biodiversité et Biotechnologie Fongiques (BBF))

  • Bernard Henrissat

    (Architecture et Fonction des Macromolécules Biologiques, UMR 7257 CNRS, USC 1408, Aix Marseille Univ
    INRAE, USC1408 Architecture et Fonction des Macromolécules Biologiques (AFMB)
    King Abdulaziz University)

  • Ditte Hededam Welner

    (The Novo Nordisk Center for Biosustainability)

  • Emma R. Master

    (University of Toronto
    Aalto University)

  • Jean-Guy Berrin

    (INRAE, Aix Marseille Univ, Biodiversité et Biotechnologie Fongiques (BBF))

  • Maher Abou Hachem

    (Technical University of Denmark)

Abstract

Oxidative plant cell-wall processing enzymes are of great importance in biology and biotechnology. Yet, our insight into the functional interplay amongst such oxidative enzymes remains limited. Here, a phylogenetic analysis of the auxiliary activity 7 family (AA7), currently harbouring oligosaccharide flavo-oxidases, reveals a striking abundance of AA7-genes in phytopathogenic fungi and Oomycetes. Expression of five fungal enzymes, including three from unexplored clades, expands the AA7-substrate range and unveils a cellooligosaccharide dehydrogenase activity, previously unknown within AA7. Sequence and structural analyses identify unique signatures distinguishing the strict dehydrogenase clade from canonical AA7 oxidases. The discovered dehydrogenase directly is able to transfer electrons to an AA9 lytic polysaccharide monooxygenase (LPMO) and fuel cellulose degradation by LPMOs without exogenous reductants. The expansion of redox-profiles and substrate range highlights the functional diversity within AA7 and sets the stage for harnessing AA7 dehydrogenases to fine-tune LPMO activity in biotechnological conversion of plant feedstocks.

Suggested Citation

  • Majid Haddad Momeni & Folmer Fredslund & Bastien Bissaro & Olanrewaju Raji & Thu V. Vuong & Sebastian Meier & Tine Sofie Nielsen & Vincent Lombard & Bruno Guigliarelli & Frédéric Biaso & Mireille Haon, 2021. "Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22372-0
    DOI: 10.1038/s41467-021-22372-0
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