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Effect of X-ray free-electron laser-induced shockwaves on haemoglobin microcrystals delivered in a liquid jet

Author

Listed:
  • Marie Luise Grünbein

    (Max Planck Institute for Medical Research, Jahnstrasse 29)

  • Alexander Gorel

    (Max Planck Institute for Medical Research, Jahnstrasse 29)

  • Lutz Foucar

    (Max Planck Institute for Medical Research, Jahnstrasse 29)

  • Sergio Carbajo

    (SLAC National Accelerator Laboratory)

  • William Colocho

    (SLAC National Accelerator Laboratory)

  • Sasha Gilevich

    (SLAC National Accelerator Laboratory)

  • Elisabeth Hartmann

    (Max Planck Institute for Medical Research, Jahnstrasse 29)

  • Mario Hilpert

    (Max Planck Institute for Medical Research, Jahnstrasse 29)

  • Mark Hunter

    (SLAC National Accelerator Laboratory)

  • Marco Kloos

    (Max Planck Institute for Medical Research, Jahnstrasse 29
    European XFEL GmbH)

  • Jason E. Koglin

    (SLAC National Accelerator Laboratory
    Los Alamos National Laboratory)

  • Thomas J. Lane

    (SLAC National Accelerator Laboratory
    Center for Free-Electron Laser Science, DESY)

  • Jim Lewandowski

    (SLAC National Accelerator Laboratory)

  • Alberto Lutman

    (SLAC National Accelerator Laboratory)

  • Karol Nass

    (Max Planck Institute for Medical Research, Jahnstrasse 29
    Paul Scherrer Institut)

  • Gabriela Nass Kovacs

    (Max Planck Institute for Medical Research, Jahnstrasse 29)

  • Christopher M. Roome

    (Max Planck Institute for Medical Research, Jahnstrasse 29)

  • John Sheppard

    (SLAC National Accelerator Laboratory)

  • Robert L. Shoeman

    (Max Planck Institute for Medical Research, Jahnstrasse 29)

  • Miriam Stricker

    (Max Planck Institute for Medical Research, Jahnstrasse 29
    University of Oxford)

  • Tim Driel

    (SLAC National Accelerator Laboratory)

  • Sharon Vetter

    (SLAC National Accelerator Laboratory)

  • R. Bruce Doak

    (Max Planck Institute for Medical Research, Jahnstrasse 29)

  • Sébastien Boutet

    (SLAC National Accelerator Laboratory)

  • Andrew Aquila

    (SLAC National Accelerator Laboratory)

  • Franz Josef Decker

    (SLAC National Accelerator Laboratory)

  • Thomas R. M. Barends

    (Max Planck Institute for Medical Research, Jahnstrasse 29)

  • Claudiu Andrei Stan

    (Rutgers University Newark)

  • Ilme Schlichting

    (Max Planck Institute for Medical Research, Jahnstrasse 29)

Abstract

X-ray free-electron lasers (XFELs) enable obtaining novel insights in structural biology. The recently available MHz repetition rate XFELs allow full data sets to be collected in shorter time and can also decrease sample consumption. However, the microsecond spacing of MHz XFEL pulses raises new challenges, including possible sample damage induced by shock waves that are launched by preceding pulses in the sample-carrying jet. We explored this matter with an X-ray-pump/X-ray-probe experiment employing haemoglobin microcrystals transported via a liquid jet into the XFEL beam. Diffraction data were collected using a shock-wave-free single-pulse scheme as well as the dual-pulse pump-probe scheme. The latter, relative to the former, reveals significant degradation of crystal hit rate, diffraction resolution and data quality. Crystal structures extracted from the two data sets also differ. Since our pump-probe attributes were chosen to emulate EuXFEL operation at its 4.5 MHz maximum pulse rate, this prompts concern about such data collection.

Suggested Citation

  • Marie Luise Grünbein & Alexander Gorel & Lutz Foucar & Sergio Carbajo & William Colocho & Sasha Gilevich & Elisabeth Hartmann & Mario Hilpert & Mark Hunter & Marco Kloos & Jason E. Koglin & Thomas J. , 2021. "Effect of X-ray free-electron laser-induced shockwaves on haemoglobin microcrystals delivered in a liquid jet," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21819-8
    DOI: 10.1038/s41467-021-21819-8
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