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Immune cell profiling of the cerebrospinal fluid enables the characterization of the brain metastasis microenvironment

Author

Listed:
  • Carlota Rubio-Perez

    (Vall d’Hebron University Hospital)

  • Ester Planas-Rigol

    (Vall d’Hebron University Hospital)

  • Juan L. Trincado

    (Barcelona Institute of Science and Technology (BIST)
    University of Barcelona)

  • Ester Bonfill-Teixidor

    (Vall d’Hebron University Hospital)

  • Alexandra Arias

    (Vall d’Hebron University Hospital)

  • Domenica Marchese

    (Barcelona Institute of Science and Technology (BIST))

  • Catia Moutinho

    (Barcelona Institute of Science and Technology (BIST))

  • Garazi Serna

    (Vall d’Hebron University Hospital)

  • Leire Pedrosa

    (University of Barcelona and Institut d’Investigacio Biomedica August Pi i Sunyer (IDIBAPS))

  • Raffaella Iurlaro

    (Vall d’Hebron University Hospital)

  • Francisco Martínez-Ricarte

    (Vall d’Hebron University Hospital
    Universitat Autònoma de Barcelona (UAB))

  • Laura Escudero

    (Vall d’Hebron University Hospital)

  • Esteban Cordero

    (Vall d’Hebron University Hospital
    Universitat Autònoma de Barcelona (UAB))

  • Marta Cicuendez

    (Vall d’Hebron University Hospital
    Universitat Autònoma de Barcelona (UAB))

  • Sara Ruiz

    (Barcelona Institute of Science and Technology (BIST))

  • Genís Parra

    (Barcelona Institute of Science and Technology (BIST))

  • Paolo Nuciforo

    (Vall d’Hebron University Hospital)

  • Josep Gonzalez

    (University of Barcelona and Institut d’Investigacio Biomedica August Pi i Sunyer (IDIBAPS))

  • Estela Pineda

    (University of Barcelona and Institut d’Investigacio Biomedica August Pi i Sunyer (IDIBAPS))

  • Juan Sahuquillo

    (Vall d’Hebron University Hospital
    Universitat Autònoma de Barcelona (UAB))

  • Josep Tabernero

    (Vall d’Hebron University Hospital
    Universitat Autònoma de Barcelona (UAB)
    CIBERONC)

  • Holger Heyn

    (Barcelona Institute of Science and Technology (BIST)
    Universitat Pompeu Fabra (UPF))

  • Joan Seoane

    (Vall d’Hebron University Hospital
    Universitat Autònoma de Barcelona (UAB)
    CIBERONC
    Institució Catalana de Recerca i Estudis Avançats (ICREA))

Abstract

Brain metastases are the most common tumor of the brain with a dismal prognosis. A fraction of patients with brain metastasis benefit from treatment with immune checkpoint inhibitors (ICI) and the degree and phenotype of the immune cell infiltration has been used to predict response to ICI. However, the anatomical location of brain lesions limits access to tumor material to characterize the immune phenotype. Here, we characterize immune cells present in brain lesions and matched cerebrospinal fluid (CSF) using single-cell RNA sequencing combined with T cell receptor genotyping. Tumor immune infiltration and specifically CD8+ T cell infiltration can be discerned through the analysis of the CSF. Consistently, identical T cell receptor clonotypes are detected in brain lesions and CSF, confirming cell exchange between these compartments. The analysis of immune cells of the CSF can provide a non-invasive alternative to predict the response to ICI, as well as identify the T cell receptor clonotypes present in brain metastasis.

Suggested Citation

  • Carlota Rubio-Perez & Ester Planas-Rigol & Juan L. Trincado & Ester Bonfill-Teixidor & Alexandra Arias & Domenica Marchese & Catia Moutinho & Garazi Serna & Leire Pedrosa & Raffaella Iurlaro & Francis, 2021. "Immune cell profiling of the cerebrospinal fluid enables the characterization of the brain metastasis microenvironment," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21789-x
    DOI: 10.1038/s41467-021-21789-x
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