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Standardization of ELISA protocols for serosurveys of the SARS-CoV-2 pandemic using clinical and at-home blood sampling

Author

Listed:
  • Carleen Klumpp-Thomas

    (National Institutes of Health
    National Institutes of Health)

  • Heather Kalish

    (National Institutes of Health)

  • Matthew Drew

    (NCI RAS Initiative, Frederick National Laboratory for Cancer Research)

  • Sally Hunsberger

    (National Institutes of Health)

  • Kelly Snead

    (NCI RAS Initiative, Frederick National Laboratory for Cancer Research)

  • Michael P. Fay

    (National Institutes of Health)

  • Jennifer Mehalko

    (NCI RAS Initiative, Frederick National Laboratory for Cancer Research)

  • Anandakumar Shunmugavel

    (National Institutes of Health)

  • Vanessa Wall

    (NCI RAS Initiative, Frederick National Laboratory for Cancer Research)

  • Peter Frank

    (NCI RAS Initiative, Frederick National Laboratory for Cancer Research)

  • John-Paul Denson

    (NCI RAS Initiative, Frederick National Laboratory for Cancer Research)

  • Min Hong

    (NCI RAS Initiative, Frederick National Laboratory for Cancer Research)

  • Gulcin Gulten

    (NCI RAS Initiative, Frederick National Laboratory for Cancer Research)

  • Simon Messing

    (NCI RAS Initiative, Frederick National Laboratory for Cancer Research)

  • Jennifer Hicks

    (National Institutes of Health)

  • Sam Michael

    (National Institutes of Health)

  • William Gillette

    (NCI RAS Initiative, Frederick National Laboratory for Cancer Research)

  • Matthew D. Hall

    (National Institutes of Health)

  • Matthew J. Memoli

    (National Institutes of Health)

  • Dominic Esposito

    (NCI RAS Initiative, Frederick National Laboratory for Cancer Research)

  • Kaitlyn Sadtler

    (National Institutes of Health)

Abstract

The extent of SARS-CoV-2 infection throughout the United States population is currently unknown. High quality serology is key to avoiding medically costly diagnostic errors, as well as to assuring properly informed public health decisions. Here, we present an optimized ELISA-based serology protocol, from antigen production to data analyses, that helps define thresholds for IgG and IgM seropositivity with high specificities. Validation of this protocol is performed using traditionally collected serum as well as dried blood on mail-in blood sampling kits. Archival (pre-2019) samples are used as negative controls, and convalescent, PCR-diagnosed COVID-19 patient samples serve as positive controls. Using this protocol, minimal cross-reactivity is observed for the spike proteins of MERS, SARS1, OC43 and HKU1 viruses, and no cross reactivity is observed with anti-influenza A H1N1 HAI. Our protocol may thus help provide standardized, population-based data on the extent of SARS-CoV-2 seropositivity, immunity and infection.

Suggested Citation

  • Carleen Klumpp-Thomas & Heather Kalish & Matthew Drew & Sally Hunsberger & Kelly Snead & Michael P. Fay & Jennifer Mehalko & Anandakumar Shunmugavel & Vanessa Wall & Peter Frank & John-Paul Denson & M, 2021. "Standardization of ELISA protocols for serosurveys of the SARS-CoV-2 pandemic using clinical and at-home blood sampling," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-020-20383-x
    DOI: 10.1038/s41467-020-20383-x
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