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Ultrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood

Author

Listed:
  • Antonio Minopoli

    (Institute of Biological Information Processing (IBI-3), Bioelectronics, Forschungszentrum Jülich
    University of Naples “Federico II”)

  • Bartolomeo Della Ventura

    (University of Naples “Federico II”)

  • Bohdan Lenyk

    (Institute of Biological Information Processing (IBI-3), Bioelectronics, Forschungszentrum Jülich
    University of Konstanz)

  • Francesco Gentile

    (University Magna Graecia)

  • Julian A. Tanner

    (University of Hong Kong)

  • Andreas Offenhäusser

    (Institute of Biological Information Processing (IBI-3), Bioelectronics, Forschungszentrum Jülich)

  • Dirk Mayer

    (Institute of Biological Information Processing (IBI-3), Bioelectronics, Forschungszentrum Jülich)

  • Raffaele Velotta

    (University of Naples “Federico II”)

Abstract

Development of plasmonic biosensors combining reliability and ease of use is still a challenge. Gold nanoparticle arrays made by block copolymer micelle nanolithography (BCMN) stand out for their scalability, cost-effectiveness and tunable plasmonic properties, making them ideal substrates for fluorescence enhancement. Here, we describe a plasmon-enhanced fluorescence immunosensor for the specific and ultrasensitive detection of Plasmodium falciparum lactate dehydrogenase (PfLDH)—a malaria marker—in whole blood. Analyte recognition is realized by oriented antibodies immobilized in a close-packed configuration via the photochemical immobilization technique (PIT), with a top bioreceptor of nucleic acid aptamers recognizing a different surface of PfLDH in a sandwich conformation. The combination of BCMN and PIT enabled maximum control over the nanoparticle size and lattice constant as well as the distance of the fluorophore from the sensing surface. The device achieved a limit of detection smaller than 1 pg/mL (

Suggested Citation

  • Antonio Minopoli & Bartolomeo Della Ventura & Bohdan Lenyk & Francesco Gentile & Julian A. Tanner & Andreas Offenhäusser & Dirk Mayer & Raffaele Velotta, 2020. "Ultrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19755-0
    DOI: 10.1038/s41467-020-19755-0
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    Cited by:

    1. Thushani Silva & Mirette Fawzy & Amirhossein Hasani & Hamidreza Ghanbari & Amin Abnavi & Abdelrahman Askar & Yue Ling & Mohammad Reza Mohammadzadeh & Fahmid Kabir & Ribwar Ahmadi & Miriam Rosin & Kare, 2022. "Ultrasensitive rapid cytokine sensors based on asymmetric geometry two-dimensional MoS2 diodes," Nature Communications, Nature, vol. 13(1), pages 1-10, December.

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