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The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans

Author

Listed:
  • Irene Sánchez-Andrea

    (Wageningen University & Research)

  • Iame Alves Guedes

    (Wageningen University & Research)

  • Bastian Hornung

    (Leids Universitair Medisch Centrum (LUMC))

  • Sjef Boeren

    (Wageningen University & Research)

  • Christopher E. Lawson

    (University of Wisconsin—Madison)

  • Diana Z. Sousa

    (Wageningen University & Research)

  • Arren Bar-Even

    (Max Planck Institute of Molecular Plant Physiology)

  • Nico J. Claassens

    (Wageningen University & Research
    Max Planck Institute of Molecular Plant Physiology)

  • Alfons J. M. Stams

    (Wageningen University & Research
    University of Minho)

Abstract

Six CO2 fixation pathways are known to operate in photoautotrophic and chemoautotrophic microorganisms. Here, we describe chemolithoautotrophic growth of the sulphate-reducing bacterium Desulfovibrio desulfuricans (strain G11) with hydrogen and sulphate as energy substrates. Genomic, transcriptomic, proteomic and metabolomic analyses reveal that D. desulfuricans assimilates CO2 via the reductive glycine pathway, a seventh CO2 fixation pathway. In this pathway, CO2 is first reduced to formate, which is reduced and condensed with a second CO2 to generate glycine. Glycine is further reduced in D. desulfuricans by glycine reductase to acetyl-P, and then to acetyl-CoA, which is condensed with another CO2 to form pyruvate. Ammonia is involved in the operation of the pathway, which is reflected in the dependence of the autotrophic growth rate on the ammonia concentration. Our study demonstrates microbial autotrophic growth fully supported by this highly ATP-efficient CO2 fixation pathway.

Suggested Citation

  • Irene Sánchez-Andrea & Iame Alves Guedes & Bastian Hornung & Sjef Boeren & Christopher E. Lawson & Diana Z. Sousa & Arren Bar-Even & Nico J. Claassens & Alfons J. M. Stams, 2020. "The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18906-7
    DOI: 10.1038/s41467-020-18906-7
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