IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-18698-w.html
   My bibliography  Save this article

Mechanism of self/nonself-discrimination in Brassica self-incompatibility

Author

Listed:
  • Kohji Murase

    (The University of Tokyo)

  • Yoshitaka Moriwaki

    (The University of Tokyo
    The University of Tokyo)

  • Tomoyuki Mori

    (Nara Institute of Science and Technology)

  • Xiao Liu

    (Nara Institute of Science and Technology)

  • Chiho Masaka

    (Nara Institute of Science and Technology)

  • Yoshinobu Takada

    (Tohoku University)

  • Ryoko Maesaki

    (Tokyo Metropolitan University)

  • Masaki Mishima

    (Tokyo Metropolitan University)

  • Sota Fujii

    (The University of Tokyo)

  • Yoshinori Hirano

    (Nara Institute of Science and Technology
    The University of Tokyo)

  • Zen Kawabe

    (The University of Tokyo)

  • Koji Nagata

    (The University of Tokyo)

  • Tohru Terada

    (The University of Tokyo
    The University of Tokyo
    The University of Tokyo)

  • Go Suzuki

    (Osaka Kyoiku University)

  • Masao Watanabe

    (Tohoku University)

  • Kentaro Shimizu

    (The University of Tokyo
    The University of Tokyo)

  • Toshio Hakoshima

    (Nara Institute of Science and Technology)

  • Seiji Takayama

    (The University of Tokyo)

Abstract

Self-incompatibility (SI) is a breeding system that promotes cross-fertilization. In Brassica, pollen rejection is induced by a haplotype-specific interaction between pistil determinant SRK (S receptor kinase) and pollen determinant SP11 (S-locus Protein 11, also named SCR) from the S-locus. Although the structure of the B. rapa S9-SRK ectodomain (eSRK) and S9-SP11 complex has been determined, it remains unclear how SRK discriminates self- and nonself-SP11. Here, we uncover the detailed mechanism of self/nonself-discrimination in Brassica SI by determining the S8-eSRK–S8-SP11 crystal structure and performing molecular dynamics (MD) simulations. Comprehensive binding analysis of eSRK and SP11 structures reveals that the binding free energies are most stable for cognate eSRK–SP11 combinations. Residue-based contribution analysis suggests that the modes of eSRK–SP11 interactions differ between intra- and inter-subgroup (a group of phylogenetically neighboring haplotypes) combinations. Our data establish a model of self/nonself-discrimination in Brassica SI.

Suggested Citation

  • Kohji Murase & Yoshitaka Moriwaki & Tomoyuki Mori & Xiao Liu & Chiho Masaka & Yoshinobu Takada & Ryoko Maesaki & Masaki Mishima & Sota Fujii & Yoshinori Hirano & Zen Kawabe & Koji Nagata & Tohru Terad, 2020. "Mechanism of self/nonself-discrimination in Brassica self-incompatibility," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18698-w
    DOI: 10.1038/s41467-020-18698-w
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-18698-w
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-18698-w?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18698-w. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.