IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-18234-w.html
   My bibliography  Save this article

A moonlighting role for enzymes of glycolysis in the co-localization of mitochondria and chloroplasts

Author

Listed:
  • Youjun Zhang

    (Max-Planck-Institut für Molekulare Pflanzenphysiologie
    Center of Plant Systems Biology and Biotechnology)

  • Arun Sampathkumar

    (Max-Planck-Institut für Molekulare Pflanzenphysiologie)

  • Sandra Mae-Lin Kerber

    (Max-Planck-Institut für Molekulare Pflanzenphysiologie)

  • Corné Swart

    (Max-Planck-Institut für Molekulare Pflanzenphysiologie)

  • Carsten Hille

    (University of Potsdam
    Technical University of Applied Sciences Wildau)

  • Kumar Seerangan

    (Max-Planck-Institut für Molekulare Pflanzenphysiologie)

  • Alexander Graf

    (Max-Planck-Institut für Molekulare Pflanzenphysiologie)

  • Lee Sweetlove

    (University of Oxford)

  • Alisdair R. Fernie

    (Max-Planck-Institut für Molekulare Pflanzenphysiologie
    Center of Plant Systems Biology and Biotechnology)

Abstract

Glycolysis is one of the primordial pathways of metabolism, playing a pivotal role in energy metabolism and biosynthesis. Glycolytic enzymes are known to form transient multi-enzyme assemblies. Here we examine the wider protein-protein interactions of plant glycolytic enzymes and reveal a moonlighting role for specific glycolytic enzymes in mediating the co-localization of mitochondria and chloroplasts. Knockout mutation of phosphoglycerate mutase or enolase resulted in a significantly reduced association of the two organelles. We provide evidence that phosphoglycerate mutase and enolase form a substrate-channelling metabolon which is part of a larger complex of proteins including pyruvate kinase. These results alongside a range of genetic complementation experiments are discussed in the context of our current understanding of chloroplast-mitochondrial interactions within photosynthetic eukaryotes.

Suggested Citation

  • Youjun Zhang & Arun Sampathkumar & Sandra Mae-Lin Kerber & Corné Swart & Carsten Hille & Kumar Seerangan & Alexander Graf & Lee Sweetlove & Alisdair R. Fernie, 2020. "A moonlighting role for enzymes of glycolysis in the co-localization of mitochondria and chloroplasts," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18234-w
    DOI: 10.1038/s41467-020-18234-w
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-18234-w
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-18234-w?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18234-w. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.