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ER-mitochondria contacts promote mtDNA nucleoids active transportation via mitochondrial dynamic tubulation

Author

Listed:
  • Jinshan Qin

    (Peking University)

  • Yuting Guo

    (Institute of Biophysics, Chinese Academy of Sciences
    College of Life Sciences, University of Chinese Academy of Sciences)

  • Boxin Xue

    (Peking University)

  • Peng Shi

    (Peking University)

  • Yang Chen

    (Peking University)

  • Qian Peter Su

    (Peking University
    University of Technology)

  • Huiwen Hao

    (Peking University)

  • Shujuan Zhao

    (Peking University)

  • Congying Wu

    (Peking University)

  • Li Yu

    (Tsinghua University)

  • Dong Li

    (Institute of Biophysics, Chinese Academy of Sciences
    College of Life Sciences, University of Chinese Academy of Sciences)

  • Yujie Sun

    (Peking University)

Abstract

A human cell contains hundreds to thousands of mitochondrial DNA (mtDNA) packaged into nucleoids. Currently, the segregation and allocation of nucleoids are thought to be passively determined by mitochondrial fusion and division. Here we provide evidence, using live-cell super-resolution imaging, that nucleoids can be actively transported via KIF5B-driven mitochondrial dynamic tubulation (MDT) activities that predominantly occur at the ER-mitochondria contact sites (EMCS). We further demonstrate that a mitochondrial inner membrane protein complex MICOS links nucleoids to Miro1, a KIF5B receptor on mitochondria, at the EMCS. We show that such active transportation is a mechanism essential for the proper distribution of nucleoids in the peripheral zone of the cell. Together, our work identifies an active transportation mechanism of nucleoids, with EMCS serving as a key platform for the interplay of nucleoids, MICOS, Miro1, and KIF5B to coordinate nucleoids segregation and transportation.

Suggested Citation

  • Jinshan Qin & Yuting Guo & Boxin Xue & Peng Shi & Yang Chen & Qian Peter Su & Huiwen Hao & Shujuan Zhao & Congying Wu & Li Yu & Dong Li & Yujie Sun, 2020. "ER-mitochondria contacts promote mtDNA nucleoids active transportation via mitochondrial dynamic tubulation," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18202-4
    DOI: 10.1038/s41467-020-18202-4
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