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A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering

Author

Listed:
  • Jiuyuan Bai

    (Sichuan University)

  • Yao Luo

    (Sichuan University)

  • Xin Wang

    (Sichuan University)

  • Shi Li

    (Sichuan University)

  • Mei Luo

    (Sichuan University)

  • Meng Yin

    (Sichuan University)

  • Yuanli Zuo

    (Sichuan University)

  • Guolin Li

    (Sichuan University)

  • Junyu Yao

    (Sichuan University)

  • Hua Yang

    (Sichuan University)

  • Mingdi Zhang

    (Sichuan University)

  • Wei Wei

    (Sichuan University)

  • Maolin Wang

    (Sichuan University)

  • Rui Wang

    (Sichuan University)

  • Chunhai Fan

    (Shanghai Jiao Tong University
    Shanghai Jiao Tong University)

  • Yun Zhao

    (Sichuan University)

Abstract

Reporter systems are routinely used in plant genetic engineering and functional genomics research. Most such plant reporter systems cause accumulation of foreign proteins. Here, we demonstrate a protein-independent reporter system, 3WJ-4 × Bro, based on a fluorescent RNA aptamer. Via transient expression assays in both Escherichia coli and Nicotiana benthamiana, we show that 3WJ-4 × Bro is suitable for transgene identification and as an mRNA reporter for expression pattern analysis. Following stable transformation in Arabidopsis thaliana, 3WJ-4 × Bro co-segregates and co-expresses with target transcripts and is stably inherited through multiple generations. Further, 3WJ-4 × Bro can be used to visualize virus-mediated RNA delivery in plants. This study demonstrates a protein-independent reporter system that can be used for transgene identification and in vivo dynamic analysis of mRNA.

Suggested Citation

  • Jiuyuan Bai & Yao Luo & Xin Wang & Shi Li & Mei Luo & Meng Yin & Yuanli Zuo & Guolin Li & Junyu Yao & Hua Yang & Mingdi Zhang & Wei Wei & Maolin Wang & Rui Wang & Chunhai Fan & Yun Zhao, 2020. "A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17497-7
    DOI: 10.1038/s41467-020-17497-7
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