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Long-term culture of human pancreatic slices as a model to study real-time islet regeneration

Author

Listed:
  • Mirza Muhammad Fahd Qadir

    (University of Miami Miller School of Medicine
    University of Miami Miller School of Medicine)

  • Silvia Álvarez-Cubela

    (University of Miami Miller School of Medicine)

  • Jonathan Weitz

    (University of Miami Miller School of Medicine)

  • Julia K. Panzer

    (University of Miami Miller School of Medicine)

  • Dagmar Klein

    (University of Miami Miller School of Medicine)

  • Yaisa Moreno-Hernández

    (University of Miami Miller School of Medicine
    Universidad Francisco de Vitoria)

  • Sirlene Cechin

    (University of Miami Miller School of Medicine)

  • Alejandro Tamayo

    (University of Miami Miller School of Medicine)

  • Joana Almaça

    (University of Miami Miller School of Medicine)

  • Helmut Hiller

    (University of Florida)

  • Maria Beery

    (University of Florida)

  • Irina Kusmartseva

    (University of Florida)

  • Mark Atkinson

    (University of Florida
    University of Florida)

  • Stephan Speier

    (Paul Langerhans Institute Dresden (PLID) of the Helmholtz Zentrum München at the University Clinic Carl Gustav Carus of Technische Universität Dresden, Helmholtz Zentrum München
    Technische Universität Dresden
    German Center for Diabetes Research (DZD))

  • Camillo Ricordi

    (University of Miami Miller School of Medicine
    University of Miami Miller School of Medicine)

  • Alberto Pugliese

    (University of Miami Miller School of Medicine
    University of Miami Miller School of Medicine)

  • Alejandro Caicedo

    (University of Miami Miller School of Medicine)

  • Christopher A. Fraker

    (University of Miami Miller School of Medicine
    University of Miami Miller School of Medicine)

  • Ricardo Luis Pastori

    (University of Miami Miller School of Medicine
    University of Miami Miller School of Medicine)

  • Juan Domínguez-Bendala

    (University of Miami Miller School of Medicine
    University of Miami Miller School of Medicine
    University of Miami Miller School of Medicine)

Abstract

The culture of live pancreatic tissue slices is a powerful tool for the interrogation of physiology and pathology in an in vitro setting that retains near-intact cytoarchitecture. However, current culture conditions for human pancreatic slices (HPSs) have only been tested for short-term applications, which are not permissive for the long-term, longitudinal study of pancreatic endocrine regeneration. Using a culture system designed to mimic the physiological oxygenation of the pancreas, we demonstrate high viability and preserved endocrine and exocrine function in HPS for at least 10 days after sectioning. This extended lifespan allowed us to dynamically lineage trace and quantify the formation of insulin-producing cells in HPS from both non-diabetic and type 2 diabetic donors. This technology is expected to be of great impact for the conduct of real-time regeneration/developmental studies in the human pancreas.

Suggested Citation

  • Mirza Muhammad Fahd Qadir & Silvia Álvarez-Cubela & Jonathan Weitz & Julia K. Panzer & Dagmar Klein & Yaisa Moreno-Hernández & Sirlene Cechin & Alejandro Tamayo & Joana Almaça & Helmut Hiller & Maria , 2020. "Long-term culture of human pancreatic slices as a model to study real-time islet regeneration," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17040-8
    DOI: 10.1038/s41467-020-17040-8
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