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The characterization of Mediator 12 and 13 as conditional positive gene regulators in Arabidopsis

Author

Listed:
  • Qikun Liu

    (Peking University
    University of California at Los Angeles)

  • Sylvain Bischof

    (University of California at Los Angeles
    University of Zurich)

  • C. Jake Harris

    (University of California at Los Angeles)

  • Zhenhui Zhong

    (University of California at Los Angeles
    Fujian Agriculture and Forestry University)

  • Lingyu Zhan

    (University of California at Los Angeles)

  • Calvin Nguyen

    (University of California at Los Angeles)

  • Andrew Rashoff

    (University of California at Los Angeles)

  • William D. Barshop

    (University of California at Los Angeles)

  • Fei Sun

    (University of California at Los Angeles)

  • Suhua Feng

    (University of California at Los Angeles)

  • Magdalena Potok

    (University of California at Los Angeles)

  • Javier Gallego-Bartolome

    (University of California at Los Angeles)

  • Jixian Zhai

    (Southern University of Science and Technology)

  • James A. Wohlschlegel

    (University of California at Los Angeles)

  • Michael F. Carey

    (University of California at Los Angeles)

  • Jeffrey A. Long

    (University of California at Los Angeles)

  • Steven E. Jacobsen

    (University of California at Los Angeles
    University of California at Los Angeles)

Abstract

Mediator 12 (MED12) and MED13 are components of the Mediator multi-protein complex, that facilitates the initial steps of gene transcription. Here, in an Arabidopsis mutant screen, we identify MED12 and MED13 as positive gene regulators, both of which contribute broadly to morc1 de-repressed gene expression. Both MED12 and MED13 are preferentially required for the expression of genes depleted in active chromatin marks, a chromatin signature shared with morc1 re-activated loci. We further discover that MED12 tends to interact with genes that are responsive to environmental stimuli, including light and radiation. We demonstrate that light-induced transient gene expression depends on MED12, and is accompanied by a concomitant increase in MED12 enrichment during induction. In contrast, the steady-state expression level of these genes show little dependence on MED12, suggesting that MED12 is primarily required to aid the expression of genes in transition from less-active to more active states.

Suggested Citation

  • Qikun Liu & Sylvain Bischof & C. Jake Harris & Zhenhui Zhong & Lingyu Zhan & Calvin Nguyen & Andrew Rashoff & William D. Barshop & Fei Sun & Suhua Feng & Magdalena Potok & Javier Gallego-Bartolome & J, 2020. "The characterization of Mediator 12 and 13 as conditional positive gene regulators in Arabidopsis," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16651-5
    DOI: 10.1038/s41467-020-16651-5
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    Cited by:

    1. Yueying Zhang & Qianli Dong & Zhen Wang & Qinzhe Liu & Haopeng Yu & Wenqing Sun & Jitender Cheema & Qiancheng You & Ling Ding & Xiaofeng Cao & Chuan He & Yiliang Ding & Huakun Zhang, 2024. "A fine-scale Arabidopsis chromatin landscape reveals chromatin conformation-associated transcriptional dynamics," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    2. Zhenhui Zhong & Yafei Wang & Ming Wang & Fan Yang & Quentin Angelo Thomas & Yan Xue & Yaxin Zhang & Wanlu Liu & Yasaman Jami-Alahmadi & Linhao Xu & Suhua Feng & Sebastian Marquardt & James A. Wohlschl, 2022. "Histone chaperone ASF1 mediates H3.3-H4 deposition in Arabidopsis," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    3. Zheng Li & Ming Wang & Zhenhui Zhong & Javier Gallego-Bartolomé & Suhua Feng & Yasaman Jami-Alahmadi & Xinyi Wang & James Wohlschlegel & Sylvain Bischof & Jeff A. Long & Steven E. Jacobsen, 2023. "The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    4. Linhua Sun & Jingru Zhou & Xiao Xu & Yi Liu & Ni Ma & Yutong Liu & Wenchao Nie & Ling Zou & Xing Wang Deng & Hang He, 2024. "Mapping nucleosome-resolution chromatin organization and enhancer-promoter loops in plants using Micro-C-XL," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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