IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-16347-w.html
   My bibliography  Save this article

The CD153 vaccine is a senotherapeutic option for preventing the accumulation of senescent T cells in mice

Author

Listed:
  • Shota Yoshida

    (Osaka University Graduate School of Medicine
    Osaka University Graduate School of Medicine)

  • Hironori Nakagami

    (Osaka University Graduate School of Medicine)

  • Hiroki Hayashi

    (Osaka University Graduate School of Medicine)

  • Yuka Ikeda

    (FunPep Co., Ltd.)

  • Jiao Sun

    (Osaka University Graduate School of Medicine)

  • Akiko Tenma

    (FunPep Co., Ltd.)

  • Hideki Tomioka

    (FunPep Co., Ltd.)

  • Tomohiro Kawano

    (Osaka University Graduate School of Medicine
    Osaka University Graduate School of Medicine)

  • Munehisa Shimamura

    (Osaka University Graduate School of Medicine)

  • Ryuichi Morishita

    (Osaka University Graduate School of Medicine)

  • Hiromi Rakugi

    (Osaka University Graduate School of Medicine)

Abstract

Senotherapy targeting for senescent cells is designed to attenuate age-related dysfunction. Senescent T cells, defined as CD4+ CD44high CD62Llow PD-1+ CD153+ cells, accumulate in visceral adipose tissues (VAT) in obese individuals. Here, we show the long-lasting effect of using CD153 vaccination to remove senescent T cells from high-fat diet (HFD)-induced obese C57BL/6J mice. We administered a CD153 peptide-KLH (keyhole limpet hemocyanin) conjugate vaccine with Alhydrogel (CD153-Alum) or CpG oligodeoxynucleotide (ODN) 1585 (CD153-CpG) and confirmed an increase in anti-CD153 antibody levels that was sustained for several months. After being fed a HFD for 10–11 weeks, adipose senescent T cell accumulation was significantly reduced in the VAT of CD153-CpG-vaccinated mice, accompanied by glucose tolerance and insulin resistance. A complement-dependent cytotoxicity (CDC) assay indicated that the mouse IgG2 antibody produced in the CD153-CpG-vaccinated mice successfully reduced the number of senescent T cells. The CD153-CpG vaccine is an optional tool for senolytic therapy.

Suggested Citation

  • Shota Yoshida & Hironori Nakagami & Hiroki Hayashi & Yuka Ikeda & Jiao Sun & Akiko Tenma & Hideki Tomioka & Tomohiro Kawano & Munehisa Shimamura & Ryuichi Morishita & Hiromi Rakugi, 2020. "The CD153 vaccine is a senotherapeutic option for preventing the accumulation of senescent T cells in mice," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16347-w
    DOI: 10.1038/s41467-020-16347-w
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-16347-w
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-16347-w?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16347-w. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.