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Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing

Author

Listed:
  • K. K. Khoo

    (University of Copenhagen)

  • I. Galleano

    (University of Copenhagen)

  • F. Gasparri

    (University of Copenhagen)

  • R. Wieneke

    (Goethe University Frankfurt)

  • H. Harms

    (University of Copenhagen)

  • M. H. Poulsen

    (University of Copenhagen)

  • H. C. Chua

    (University of Copenhagen)

  • M. Wulf

    (University of Copenhagen)

  • R. Tampé

    (Goethe University Frankfurt)

  • S. A. Pless

    (University of Copenhagen)

Abstract

Manipulation of proteins by chemical modification is a powerful way to decipher their function. However, most ribosome-dependent and semi-synthetic methods have limitations in the number and type of modifications that can be introduced, especially in live cells. Here, we present an approach to incorporate single or multiple post-translational modifications or non-canonical amino acids into proteins expressed in eukaryotic cells. We insert synthetic peptides into GFP, NaV1.5 and P2X2 receptors via tandem protein trans-splicing using two orthogonal split intein pairs and validate our approach by investigating protein function. We anticipate the approach will overcome some drawbacks of existing protein enigineering methods.

Suggested Citation

  • K. K. Khoo & I. Galleano & F. Gasparri & R. Wieneke & H. Harms & M. H. Poulsen & H. C. Chua & M. Wulf & R. Tampé & S. A. Pless, 2020. "Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16208-6
    DOI: 10.1038/s41467-020-16208-6
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