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Neutralization of SARS-CoV-2 spike pseudotyped virus by recombinant ACE2-Ig

Author

Listed:
  • Changhai Lei

    (Department of Biophysics, College of Basic Medical Sciences, Second Military Medical University
    Team SMMU-China of the International Genetically Engineered Machine (iGEM) competition, Department of Biophysics, Second Military Medical University)

  • Kewen Qian

    (Department of Biophysics, College of Basic Medical Sciences, Second Military Medical University
    Team SMMU-China of the International Genetically Engineered Machine (iGEM) competition, Department of Biophysics, Second Military Medical University)

  • Tian Li

    (Department of Biophysics, College of Basic Medical Sciences, Second Military Medical University
    Team SMMU-China of the International Genetically Engineered Machine (iGEM) competition, Department of Biophysics, Second Military Medical University)

  • Sheng Zhang

    (Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine)

  • Wenyan Fu

    (Department of Assisted Reproduction, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine)

  • Min Ding

    (Pharchoice Therapeutics, Inc)

  • Shi Hu

    (Department of Biophysics, College of Basic Medical Sciences, Second Military Medical University
    Team SMMU-China of the International Genetically Engineered Machine (iGEM) competition, Department of Biophysics, Second Military Medical University)

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in Wuhan, China, at the end of 2019, and there are currently no specific antiviral treatments or vaccines available. SARS-CoV-2 has been shown to use the same cell entry receptor as SARS-CoV, angiotensin-converting enzyme 2 (ACE2). In this report, we generate a recombinant protein by connecting the extracellular domain of human ACE2 to the Fc region of the human immunoglobulin IgG1. A fusion protein containing an ACE2 mutant with low catalytic activity is also used in this study. The fusion proteins are then characterized. Both fusion proteins have a high binding affinity for the receptor-binding domains of SARS-CoV and SARS-CoV-2 and exhibit desirable pharmacological properties in mice. Moreover, the fusion proteins neutralize virus pseudotyped with SARS-CoV or SARS-CoV-2 spike proteins in vitro. As these fusion proteins exhibit cross-reactivity against coronaviruses, they have potential applications in the diagnosis, prophylaxis, and treatment of SARS-CoV-2.

Suggested Citation

  • Changhai Lei & Kewen Qian & Tian Li & Sheng Zhang & Wenyan Fu & Min Ding & Shi Hu, 2020. "Neutralization of SARS-CoV-2 spike pseudotyped virus by recombinant ACE2-Ig," Nature Communications, Nature, vol. 11(1), pages 1-5, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16048-4
    DOI: 10.1038/s41467-020-16048-4
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