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Chromosome organization by a conserved condensin-ParB system in the actinobacterium Corynebacterium glutamicum

Author

Listed:
  • Kati Böhm

    (Ludwig-Maximilians-Universität München, Fakultät Biologie)

  • Giacomo Giacomelli

    (Ludwig-Maximilians-Universität München, Fakultät Biologie
    Christian-Albrechts-Universität zu Kiel, Institut für allgemeine Mikrobiologie)

  • Andreas Schmidt

    (Biomedical Center, Protein Analysis Unit, Faculty of Medicine, Ludwig-Maximilians-Universität München)

  • Axel Imhof

    (Biomedical Center, Protein Analysis Unit, Faculty of Medicine, Ludwig-Maximilians-Universität München)

  • Romain Koszul

    (Institut Pasteur, Unité Régulation Spatiales des Génomes, UMR3525, CNRS
    Institut Pasteur, Center of Bioinformatics, Biostatistics and Integrative Biology (C3BI))

  • Martial Marbouty

    (Institut Pasteur, Unité Régulation Spatiales des Génomes, UMR3525, CNRS)

  • Marc Bramkamp

    (Ludwig-Maximilians-Universität München, Fakultät Biologie
    Christian-Albrechts-Universität zu Kiel, Institut für allgemeine Mikrobiologie)

Abstract

Higher-order chromosome folding and segregation are tightly regulated in all domains of life. In bacteria, details on nucleoid organization regulatory mechanisms and function remain poorly characterized, especially in non-model species. Here, we investigate the role of DNA-partitioning protein ParB and SMC condensin complexes in the actinobacterium Corynebacterium glutamicum. Chromosome conformation capture reveals SMC-mediated long-range interactions around ten centromere-like parS sites clustered at the replication origin (oriC). At least one oriC-proximal parS site is necessary for reliable chromosome segregation. We use chromatin immunoprecipitation and photoactivated single-molecule localization microscopy to show the formation of distinct, parS-dependent ParB-nucleoprotein subclusters. We further show that SMC/ScpAB complexes, loaded via ParB at parS sites, mediate chromosomal inter-arm contacts (as previously shown in Bacillus subtilis). However, the MukBEF-like SMC complex MksBEFG does not contribute to chromosomal DNA-folding; instead, this complex is involved in plasmid maintenance and interacts with the polar oriC-tethering factor DivIVA. Our results complement current models of ParB-SMC/ScpAB crosstalk and show that some condensin complexes evolved functions that are apparently uncoupled from chromosome folding.

Suggested Citation

  • Kati Böhm & Giacomo Giacomelli & Andreas Schmidt & Axel Imhof & Romain Koszul & Martial Marbouty & Marc Bramkamp, 2020. "Chromosome organization by a conserved condensin-ParB system in the actinobacterium Corynebacterium glutamicum," Nature Communications, Nature, vol. 11(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15238-4
    DOI: 10.1038/s41467-020-15238-4
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    Cited by:

    1. Qin Liao & Hugo B. Brandão & Zhongqing Ren & Xindan Wang, 2025. "Replisomes restrict SMC translocation in vivo," Nature Communications, Nature, vol. 16(1), pages 1-18, December.
    2. Constantin N. Takacs & Jenny Wachter & Yingjie Xiang & Zhongqing Ren & Xheni Karaboja & Molly Scott & Matthew R. Stoner & Irnov Irnov & Nicholas Jannetty & Patricia A. Rosa & Xindan Wang & Christine J, 2022. "Polyploidy, regular patterning of genome copies, and unusual control of DNA partitioning in the Lyme disease spirochete," Nature Communications, Nature, vol. 13(1), pages 1-22, December.

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