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Herpes simplex virus blocks host transcription termination via the bimodal activities of ICP27

Author

Listed:
  • Xiuye Wang

    (University of California, Irvine)

  • Thomas Hennig

    (Julius-Maximilians-University Würzburg)

  • Adam W. Whisnant

    (Julius-Maximilians-University Würzburg)

  • Florian Erhard

    (Julius-Maximilians-University Würzburg)

  • Bhupesh K. Prusty

    (Julius-Maximilians-University Würzburg)

  • Caroline C. Friedel

    (Ludwig-Maximilians-Universität München)

  • Elmira Forouzmand

    (University of California, Irvine
    University of California, Irvine)

  • William Hu

    (University of California, Irvine)

  • Luke Erber

    (University of Minnesota)

  • Yue Chen

    (University of Minnesota)

  • Rozanne M. Sandri-Goldin

    (University of California, Irvine)

  • Lars Dölken

    (Julius-Maximilians-University Würzburg
    Helmholtz Institute for RNA-based Infection Research)

  • Yongsheng Shi

    (University of California, Irvine)

Abstract

Infection by viruses, including herpes simplex virus-1 (HSV-1), and cellular stresses cause widespread disruption of transcription termination (DoTT) of RNA polymerase II (RNAPII) in host genes. However, the underlying mechanisms remain unclear. Here, we demonstrate that the HSV-1 immediate early protein ICP27 induces DoTT by directly binding to the essential mRNA 3’ processing factor CPSF. It thereby induces the assembly of a dead-end 3’ processing complex, blocking mRNA 3’ cleavage. Remarkably, ICP27 also acts as a sequence-dependent activator of mRNA 3’ processing for viral and a subset of host transcripts. Our results unravel a bimodal activity of ICP27 that plays a key role in HSV-1-induced host shutoff and identify CPSF as an important factor that mediates regulation of transcription termination. These findings have broad implications for understanding the regulation of transcription termination by other viruses, cellular stress and cancer.

Suggested Citation

  • Xiuye Wang & Thomas Hennig & Adam W. Whisnant & Florian Erhard & Bhupesh K. Prusty & Caroline C. Friedel & Elmira Forouzmand & William Hu & Luke Erber & Yue Chen & Rozanne M. Sandri-Goldin & Lars Dölk, 2020. "Herpes simplex virus blocks host transcription termination via the bimodal activities of ICP27," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-14109-x
    DOI: 10.1038/s41467-019-14109-x
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    Cited by:

    1. Lara Djakovic & Thomas Hennig & Katharina Reinisch & Andrea Milić & Adam W. Whisnant & Katharina Wolf & Elena Weiß & Tobias Haas & Arnhild Grothey & Christopher S. Jürges & Michael Kluge & Elmar Wolf , 2023. "The HSV-1 ICP22 protein selectively impairs histone repositioning upon Pol II transcription downstream of genes," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    2. Yange Cui & Luyang Wang & Qingbao Ding & Jihae Shin & Joel Cassel & Qin Liu & Joseph M. Salvino & Bin Tian, 2023. "Elevated pre-mRNA 3′ end processing activity in cancer cells renders vulnerability to inhibition of cleavage and polyadenylation," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    3. Buki Kwon & Mervin M. Fansler & Neil D. Patel & Jihye Lee & Weirui Ma & Christine Mayr, 2022. "Enhancers regulate 3′ end processing activity to control expression of alternative 3′UTR isoforms," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    4. Valter Bergant & Daniel Schnepf & Niklas Andrade Krätzig & Philipp Hubel & Christian Urban & Thomas Engleitner & Ronald Dijkman & Bernhard Ryffel & Katja Steiger & Percy A. Knolle & Georg Kochs & Rola, 2023. "mRNA 3’UTR lengthening by alternative polyadenylation attenuates inflammatory responses and correlates with virulence of Influenza A virus," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

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