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Image quality guided smart rotation improves coverage in microscopy

Author

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  • Jiaye He

    (Morgridge Institute for Research
    Max Planck Institute for Molecular Cell Biology and Genetics
    University of Wisconsin Madison)

  • Jan Huisken

    (Morgridge Institute for Research
    University of Wisconsin Madison)

Abstract

Fluorescence microscopy is an essential tool for biological discoveries. There is a constant demand for better spatial resolution across a larger field of view. Although strides have been made to improve the theoretical resolution and speed of the optical instruments, in mesoscopic samples, image quality is still largely limited by the optical properties of the sample. In Selective Plane Illumination Microscopy (SPIM), the achievable optical performance is hampered by optical degradations encountered in both the illumination and detection. Multi-view imaging, either through sample rotation or additional optical paths, is a popular strategy to improve sample coverage. In this work, we introduce a smart rotation workflow that utilizes on-the-fly image analysis to identify the optimal light sheet imaging orientations. The smart rotation workflow outperforms the conventional approach without additional hardware and achieves a better sample coverage using the same number of angles or less and thereby reduces data volume and phototoxicity.

Suggested Citation

  • Jiaye He & Jan Huisken, 2020. "Image quality guided smart rotation improves coverage in microscopy," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-13821-y
    DOI: 10.1038/s41467-019-13821-y
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