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An artificial metalloenzyme biosensor can detect ethylene gas in fruits and Arabidopsis leaves

Author

Listed:
  • Kenward Vong

    (RIKEN Cluster for Pioneering Research)

  • Shohei Eda

    (RIKEN Cluster for Pioneering Research
    RIKEN Baton Zone Program)

  • Yasuhiro Kadota

    (RIKEN Center for Sustainable Resource Science)

  • Igor Nasibullin

    (RIKEN Cluster for Pioneering Research)

  • Takanori Wakatake

    (RIKEN Center for Sustainable Resource Science)

  • Satoshi Yokoshima

    (Nagoya University, Furo-cho)

  • Ken Shirasu

    (RIKEN Center for Sustainable Resource Science)

  • Katsunori Tanaka

    (RIKEN Cluster for Pioneering Research
    RIKEN Baton Zone Program
    Kazan Federal University
    Tokyo Institute of Technology)

Abstract

Enzyme biosensors are useful tools that can monitor rapid changes in metabolite levels in real-time. However, current approaches are largely constrained to metabolites within a limited chemical space. With the rising development of artificial metalloenzymes (ArM), a unique opportunity exists to design biosensors from the ground-up for metabolites that are difficult to detect using current technologies. Here we present the design and development of the ArM ethylene probe (AEP), where an albumin scaffold is used to solubilize and protect a quenched ruthenium catalyst. In the presence of the phytohormone ethylene, cross metathesis can occur to produce fluorescence. The probe can be used to detect both exogenous- and endogenous-induced changes to ethylene biosynthesis in fruits and leaves. Overall, this work represents an example of an ArM biosensor, designed specifically for the spatial and temporal detection of a biological metabolite previously not accessible using enzyme biosensors.

Suggested Citation

  • Kenward Vong & Shohei Eda & Yasuhiro Kadota & Igor Nasibullin & Takanori Wakatake & Satoshi Yokoshima & Ken Shirasu & Katsunori Tanaka, 2019. "An artificial metalloenzyme biosensor can detect ethylene gas in fruits and Arabidopsis leaves," Nature Communications, Nature, vol. 10(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13758-2
    DOI: 10.1038/s41467-019-13758-2
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