IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-12942-8.html
   My bibliography  Save this article

Structure of a cyanobacterial photosystem I tetramer revealed by cryo-electron microscopy

Author

Listed:
  • Koji Kato

    (Okayama University)

  • Ryo Nagao

    (Okayama University)

  • Tian-Yi Jiang

    (Okayama University)

  • Yoshifumi Ueno

    (Kobe University)

  • Makio Yokono

    (Nippon Flour Mills Co., Ltd., Innovation Center)

  • Siu Kit Chan

    (Okayama University)

  • Mai Watanabe

    (The University of Tokyo)

  • Masahiko Ikeuchi

    (The University of Tokyo)

  • Jian-Ren Shen

    (Okayama University)

  • Seiji Akimoto

    (Kobe University)

  • Naoyuki Miyazaki

    (Tsukuba Advanced Research Alliance (TARA), University of Tsukuba
    Osaka University)

  • Fusamichi Akita

    (Okayama University
    Japan Science and Technology Agency, PRESTO)

Abstract

Photosystem I (PSI) functions to harvest light energy for conversion into chemical energy. The organisation of PSI is variable depending on the species of organism. Here we report the structure of a tetrameric PSI core isolated from a cyanobacterium, Anabaena sp. PCC 7120, analysed by single-particle cryo-electron microscopy (cryo-EM) at 3.3 Å resolution. The PSI tetramer has a C2 symmetry and is organised in a dimer of dimers form. The structure reveals interactions at the dimer-dimer interface and the existence of characteristic pigment orientations and inter-pigment distances within the dimer units that are important for unique excitation energy transfer. In particular, characteristic residues of PsaL are identified to be responsible for the formation of the tetramer. Time-resolved fluorescence analyses showed that the PSI tetramer has an enhanced excitation-energy quenching. These structural and spectroscopic findings provide insights into the physiological significance of the PSI tetramer and evolutionary changes of the PSI organisations.

Suggested Citation

  • Koji Kato & Ryo Nagao & Tian-Yi Jiang & Yoshifumi Ueno & Makio Yokono & Siu Kit Chan & Mai Watanabe & Masahiko Ikeuchi & Jian-Ren Shen & Seiji Akimoto & Naoyuki Miyazaki & Fusamichi Akita, 2019. "Structure of a cyanobacterial photosystem I tetramer revealed by cryo-electron microscopy," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12942-8
    DOI: 10.1038/s41467-019-12942-8
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-12942-8
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-12942-8?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Ryo Nagao & Koji Kato & Tasuku Hamaguchi & Yoshifumi Ueno & Naoki Tsuboshita & Shota Shimizu & Miyu Furutani & Shigeki Ehira & Yoshiki Nakajima & Keisuke Kawakami & Takehiro Suzuki & Naoshi Dohmae & S, 2023. "Structure of a monomeric photosystem I core associated with iron-stress-induced-A proteins from Anabaena sp. PCC 7120," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Koji Kato & Ryo Nagao & Yoshifumi Ueno & Makio Yokono & Takehiro Suzuki & Tian-Yi Jiang & Naoshi Dohmae & Fusamichi Akita & Seiji Akimoto & Naoyuki Miyazaki & Jian-Ren Shen, 2022. "Structure of a tetrameric photosystem I from a glaucophyte alga Cyanophora paradoxa," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    3. Shivam Yadav & Martin Centola & Mathilda Glaesmann & Denys Pogoryelov & Roman Ladig & Mike Heilemann & L. C. Rai & Özkan Yildiz & Enrico Schleiff, 2022. "Cyclophilin anaCyp40 regulates photosystem assembly and phycobilisome association in a cyanobacterium," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    4. Ziyu Zhao & Irene Vercellino & Jana Knoppová & Roman Sobotka & James W. Murray & Peter J. Nixon & Leonid A. Sazanov & Josef Komenda, 2023. "The Ycf48 accessory factor occupies the site of the oxygen-evolving manganese cluster during photosystem II biogenesis," Nature Communications, Nature, vol. 14(1), pages 1-11, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12942-8. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.