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Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension

Author

Listed:
  • Matteo Biancospino

    (IFOM, Fondazione Istituto FIRC di Oncologia Molecolare)

  • Gwen R. Buel

    (National Cancer Institute)

  • Carlos A. Niño

    (IFOM, Fondazione Istituto FIRC di Oncologia Molecolare)

  • Elena Maspero

    (IFOM, Fondazione Istituto FIRC di Oncologia Molecolare)

  • Rossella Scotto di Perrotolo

    (IFOM, Fondazione Istituto FIRC di Oncologia Molecolare)

  • Andrea Raimondi

    (San Raffaele Scientific Institute)

  • Lisa Redlingshöfer

    (University College London)

  • Janine Weber

    (IFOM, Fondazione Istituto FIRC di Oncologia Molecolare)

  • Frances M. Brodsky

    (University College London)

  • Kylie J. Walters

    (National Cancer Institute)

  • Simona Polo

    (IFOM, Fondazione Istituto FIRC di Oncologia Molecolare
    Universita’ degli Studi di Milano)

Abstract

Clathrin light chains (CLCa and CLCb) are major constituents of clathrin-coated vesicles. Unique functions for these evolutionary conserved paralogs remain elusive, and their role in clathrin-mediated endocytosis in mammalian cells is debated. Here, we find and structurally characterize a direct and selective interaction between CLCa and the long isoform of the actin motor protein myosin VI, which is expressed exclusively in highly polarized tissues. Using genetically-reconstituted Caco-2 cysts as proxy for polarized epithelia, we provide evidence for coordinated action of myosin VI and CLCa at the apical surface where these proteins are essential for fission of clathrin-coated pits. We further find that myosin VI and Huntingtin-interacting protein 1-related protein (Hip1R) are mutually exclusive interactors with CLCa, and suggest a model for the sequential function of myosin VI and Hip1R in actin-mediated clathrin-coated vesicle budding.

Suggested Citation

  • Matteo Biancospino & Gwen R. Buel & Carlos A. Niño & Elena Maspero & Rossella Scotto di Perrotolo & Andrea Raimondi & Lisa Redlingshöfer & Janine Weber & Frances M. Brodsky & Kylie J. Walters & Simona, 2019. "Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension," Nature Communications, Nature, vol. 10(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12855-6
    DOI: 10.1038/s41467-019-12855-6
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    Cited by:

    1. Fengfeng Niu & Lingxuan Li & Lei Wang & Jinman Xiao & Shun Xu & Yong Liu & Leishu Lin & Cong Yu & Zhiyi Wei, 2024. "Autoinhibition and activation of myosin VI revealed by its cryo-EM structure," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    2. Kazuki Obashi & Kem A. Sochacki & Marie-Paule Strub & Justin W. Taraska, 2023. "A conformational switch in clathrin light chain regulates lattice structure and endocytosis at the plasma membrane of mammalian cells," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

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