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FIERY1 promotes microRNA accumulation by suppressing rRNA-derived small interfering RNAs in Arabidopsis

Author

Listed:
  • Chenjiang You

    (Shenzhen University
    Shenzhen University
    University of California)

  • Wenrong He

    (University of California)

  • Runlai Hang

    (Shenzhen University
    University of California)

  • Cuiju Zhang

    (Shenzhen University)

  • Xiaofeng Cao

    (Chinese Academy of Sciences)

  • Hongwei Guo

    (Southern University of Science and Technology of China)

  • Xuemei Chen

    (University of California)

  • Jie Cui

    (Shenzhen University)

  • Beixin Mo

    (Shenzhen University)

Abstract

Plant microRNAs (miRNAs) associate with ARGONAUTE1 (AGO1) to direct post-transcriptional gene silencing and regulate numerous biological processes. Although AGO1 predominantly binds miRNAs in vivo, it also associates with endogenous small interfering RNAs (siRNAs). It is unclear whether the miRNA/siRNA balance affects miRNA activities. Here we report that FIERY1 (FRY1), which is involved in 5′−3′ RNA degradation, regulates miRNA abundance and function by suppressing the biogenesis of ribosomal RNA-derived siRNAs (risiRNAs). In mutants of FRY1 and the nuclear 5′−3′ exonuclease genes XRN2 and XRN3, we find that a large number of 21-nt risiRNAs are generated through an endogenous siRNA biogenesis pathway. The production of risiRNAs correlates with pre-rRNA processing defects in these mutants. We also show that these risiRNAs are loaded into AGO1, causing reduced loading of miRNAs. This study reveals a previously unknown link between rRNA processing and miRNA accumulation.

Suggested Citation

  • Chenjiang You & Wenrong He & Runlai Hang & Cuiju Zhang & Xiaofeng Cao & Hongwei Guo & Xuemei Chen & Jie Cui & Beixin Mo, 2019. "FIERY1 promotes microRNA accumulation by suppressing rRNA-derived small interfering RNAs in Arabidopsis," Nature Communications, Nature, vol. 10(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12379-z
    DOI: 10.1038/s41467-019-12379-z
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