IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-12080-1.html

Some searches may not work properly. We apologize for the inconvenience.

   My bibliography  Save this article

Enhancing and shaping the immunogenicity of native-like HIV-1 envelope trimers with a two-component protein nanoparticle

Author

Listed:
  • Philip J. M. Brouwer

    (University of Amsterdam)

  • Aleksandar Antanasijevic

    (The Scripps Research Institute)

  • Zachary Berndsen

    (The Scripps Research Institute)

  • Anila Yasmeen

    (Weill Medical College of Cornell University)

  • Brooke Fiala

    (University of Washington
    University of Washington)

  • Tom P. L. Bijl

    (University of Amsterdam)

  • Ilja Bontjer

    (University of Amsterdam)

  • Jacob B. Bale

    (University of Washington
    University of Washington
    Arzeda Corporation)

  • William Sheffler

    (University of Washington
    University of Washington)

  • Joel D. Allen

    (University of Southampton)

  • Anna Schorcht

    (University of Amsterdam)

  • Judith A. Burger

    (University of Amsterdam)

  • Miguel Camacho

    (University of Amsterdam)

  • Daniel Ellis

    (University of Washington
    University of Washington)

  • Christopher A. Cottrell

    (The Scripps Research Institute)

  • Anna-Janina Behrens

    (University of Oxford
    New England Biolabs, Inc.)

  • Marco Catalano

    (University of Amsterdam)

  • Iván del Moral-Sánchez

    (University of Amsterdam)

  • Thomas J. Ketas

    (Weill Medical College of Cornell University)

  • Celia LaBranche

    (Duke University Medical Center)

  • Marit J. van Gils

    (University of Amsterdam)

  • Kwinten Sliepen

    (University of Amsterdam)

  • Lance J. Stewart

    (University of Washington
    University of Washington)

  • Max Crispin

    (University of Southampton
    University of Oxford)

  • David C. Montefiori

    (Duke University Medical Center)

  • David Baker

    (University of Washington
    University of Washington
    University of Washington)

  • John P. Moore

    (Weill Medical College of Cornell University)

  • Per Johan Klasse

    (Weill Medical College of Cornell University)

  • Andrew B. Ward

    (The Scripps Research Institute)

  • Neil P. King

    (University of Washington
    University of Washington)

  • Rogier W. Sanders

    (University of Amsterdam)

Abstract

The development of native-like HIV-1 envelope (Env) trimer antigens has enabled the induction of neutralizing antibody (NAb) responses against neutralization-resistant HIV-1 strains in animal models. However, NAb responses are relatively weak and narrow in specificity. Displaying antigens in a multivalent fashion on nanoparticles (NPs) is an established strategy to increase their immunogenicity. Here we present the design and characterization of two-component protein NPs displaying 20 stabilized SOSIP trimers from various HIV-1 strains. The two-component nature permits the incorporation of exclusively well-folded, native-like Env trimers into NPs that self-assemble in vitro with high efficiency. Immunization studies show that the NPs are particularly efficacious as priming immunogens, improve the quality of the Ab response over a conventional one-component nanoparticle system, and are most effective when SOSIP trimers with an apex-proximate neutralizing epitope are displayed. Their ability to enhance and shape the immunogenicity of SOSIP trimers make these NPs a promising immunogen platform.

Suggested Citation

  • Philip J. M. Brouwer & Aleksandar Antanasijevic & Zachary Berndsen & Anila Yasmeen & Brooke Fiala & Tom P. L. Bijl & Ilja Bontjer & Jacob B. Bale & William Sheffler & Joel D. Allen & Anna Schorcht & J, 2019. "Enhancing and shaping the immunogenicity of native-like HIV-1 envelope trimers with a two-component protein nanoparticle," Nature Communications, Nature, vol. 10(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12080-1
    DOI: 10.1038/s41467-019-12080-1
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-12080-1
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-12080-1?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12080-1. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.