IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-11963-7.html
   My bibliography  Save this article

Misshapen coordinates protrusion restriction and actomyosin contractility during collective cell migration

Author

Listed:
  • Cédric Plutoni

    (Université de Montréal)

  • Sarah Keil

    (Université de Montréal)

  • Carlos Zeledon

    (Université de Montréal)

  • Lara Elis Alberici Delsin

    (Université de Montréal)

  • Barbara Decelle

    (Université de Montréal)

  • Philippe P. Roux

    (Université de Montréal
    Université de Montréal)

  • Sébastien Carréno

    (Université de Montréal
    Université de Montréal)

  • Gregory Emery

    (Université de Montréal
    Université de Montréal)

Abstract

Collective cell migration is involved in development, wound healing and metastasis. In the Drosophila ovary, border cells (BC) form a small cluster that migrates collectively through the egg chamber. To achieve directed motility, the BC cluster coordinates the formation of protrusions in its leader cell and contractility at the rear. Restricting protrusions to leader cells requires the actin and plasma membrane linker Moesin. Herein, we show that the Ste20-like kinase Misshapen phosphorylates Moesin in vitro and in BC. Depletion of Misshapen disrupts protrusion restriction, thereby allowing other cells within the cluster to protrude. In addition, we show that Misshapen is critical to generate contractile forces both at the rear of the cluster and at the base of protrusions. Together, our results indicate that Misshapen is a key regulator of BC migration as it coordinates two independent pathways that restrict protrusion formation to the leader cells and induces contractile forces.

Suggested Citation

  • Cédric Plutoni & Sarah Keil & Carlos Zeledon & Lara Elis Alberici Delsin & Barbara Decelle & Philippe P. Roux & Sébastien Carréno & Gregory Emery, 2019. "Misshapen coordinates protrusion restriction and actomyosin contractility during collective cell migration," Nature Communications, Nature, vol. 10(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11963-7
    DOI: 10.1038/s41467-019-11963-7
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-11963-7
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-11963-7?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11963-7. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.