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Distinct methylation levels of mature microRNAs in gastrointestinal cancers

Author

Listed:
  • Masamitsu Konno

    (Osaka University)

  • Jun Koseki

    (Osaka University)

  • Ayumu Asai

    (Osaka University
    Osaka University)

  • Akira Yamagata

    (Idea Consultants, Inc., Osaka-city)

  • Teppei Shimamura

    (Nagoya University Graduate School of Medicine)

  • Daisuke Motooka

    (Osaka University)

  • Daisuke Okuzaki

    (Osaka University)

  • Koichi Kawamoto

    (Osaka University)

  • Tsunekazu Mizushima

    (Osaka University)

  • Hidetoshi Eguchi

    (Osaka University)

  • Shuji Takiguchi

    (Osaka University
    Nagoya City University Graduate School of Medical Sciences and Medical School)

  • Taroh Satoh

    (Osaka University)

  • Koshi Mimori

    (Kyushu University Beppu Hospital)

  • Takahiro Ochiya

    (National Cancer Center Research Institute)

  • Yuichiro Doki

    (Osaka University)

  • Ken Ofusa

    (Idea Consultants, Inc., Osaka-city)

  • Masaki Mori

    (Osaka University)

  • Hideshi Ishii

    (Osaka University)

Abstract

The biological significance of micro (mi)RNAs has traditionally been evaluated according to their RNA expression levels based on the assumption that miRNAs recognize and regulate their targets in an unvarying fashion. Here we show that a fraction of mature miRNAs including miR-17-5p, -21-5p, and -200c-3p and let-7a-5p harbor methyl marks that potentially alter their stability and target recognition. Importantly, methylation of these miRNAs was significantly increased in cancer tissues as compared to paired normal tissues. Furthermore, miR-17-5p methylation level in serum samples distinguished early pancreatic cancer patients from healthy controls with extremely high sensitivity and specificity. These findings provide a basis for diagnostic strategies for early-stage cancer and add a dimension to our understanding of miRNA biology.

Suggested Citation

  • Masamitsu Konno & Jun Koseki & Ayumu Asai & Akira Yamagata & Teppei Shimamura & Daisuke Motooka & Daisuke Okuzaki & Koichi Kawamoto & Tsunekazu Mizushima & Hidetoshi Eguchi & Shuji Takiguchi & Taroh S, 2019. "Distinct methylation levels of mature microRNAs in gastrointestinal cancers," Nature Communications, Nature, vol. 10(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11826-1
    DOI: 10.1038/s41467-019-11826-1
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