IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-11356-w.html
   My bibliography  Save this article

Time-resolved NMR monitoring of tRNA maturation

Author

Listed:
  • Pierre Barraud

    (Institut de biologie physico-chimique)

  • Alexandre Gato

    (Institut de biologie physico-chimique)

  • Matthias Heiss

    (Ludwig Maximilians University Munich)

  • Marjorie Catala

    (Institut de biologie physico-chimique)

  • Stefanie Kellner

    (Ludwig Maximilians University Munich)

  • Carine Tisné

    (Institut de biologie physico-chimique)

Abstract

Although the biological importance of post-transcriptional RNA modifications in gene expression is widely appreciated, methods to directly detect their introduction during RNA biosynthesis are rare and do not easily provide information on the temporal nature of events. Here, we introduce the application of NMR spectroscopy to observe the maturation of tRNAs in cell extracts. By following the maturation of yeast tRNAPhe with time-resolved NMR measurements, we show that modifications are introduced in a defined sequential order, and that the chronology is controlled by cross-talk between modification events. In particular, we show that a strong hierarchy controls the introduction of the T54, Ψ55 and m1A58 modifications in the T-arm, and we demonstrate that the modification circuits identified in yeast extract with NMR also impact the tRNA modification process in living cells. The NMR-based methodology presented here could be adapted to investigate different aspects of tRNA maturation and RNA modifications in general.

Suggested Citation

  • Pierre Barraud & Alexandre Gato & Matthias Heiss & Marjorie Catala & Stefanie Kellner & Carine Tisné, 2019. "Time-resolved NMR monitoring of tRNA maturation," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11356-w
    DOI: 10.1038/s41467-019-11356-w
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-11356-w
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-11356-w?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Gabrielle Bourgeois & Pierre-Damien Coureux & Christine Lazennec-Schurdevin & Clément Madru & Thomas Gaillard & Magalie Duchateau & Julia Chamot-Rooke & Sophie Bourcier & Yves Mechulam & Emmanuelle Sc, 2025. "Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity," Nature Communications, Nature, vol. 16(1), pages 1-18, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11356-w. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.