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Live cell imaging reveals 3′-UTR dependent mRNA sorting to synapses

Author

Listed:
  • Karl E. Bauer

    (Ludwig Maximilians University)

  • Inmaculada Segura

    (Ludwig Maximilians University)

  • Imre Gaspar

    (EMBL
    Institute of Molecular Biotechnology)

  • Volker Scheuss

    (Ludwig Maximilians University)

  • Christin Illig

    (Ludwig Maximilians University)

  • Georg Ammer

    (Ludwig Maximilians University
    MPI of Neurobiology)

  • Saskia Hutten

    (Ludwig Maximilians University)

  • Eugénia Basyuk

    (CNRS UMR5535, Université de Montpellier
    CNRS UMR9002, Université de Montpellier)

  • Sandra M. Fernández-Moya

    (Ludwig Maximilians University)

  • Janina Ehses

    (Ludwig Maximilians University)

  • Edouard Bertrand

    (CNRS UMR5535, Université de Montpellier)

  • Michael A. Kiebler

    (Ludwig Maximilians University)

Abstract

mRNA transport restricts translation to specific subcellular locations, which is the basis for many cellular functions. However, the precise process of mRNA sorting to synapses in neurons remains elusive. Here we use Rgs4 mRNA to investigate 3′-UTR-dependent transport by MS2 live-cell imaging. The majority of observed RNA granules display 3′-UTR independent bidirectional transport in dendrites. Importantly, the Rgs4 3′-UTR causes an anterograde transport bias, which requires the Staufen2 protein. Moreover, the 3′-UTR mediates dynamic, sustained mRNA recruitment to synapses. Visualization at high temporal resolution enables us to show mRNA patrolling dendrites, allowing transient interaction with multiple synapses, in agreement with the sushi-belt model. Modulation of neuronal activity by either chemical silencing or local glutamate uncaging regulates both the 3′-UTR-dependent transport bias and synaptic recruitment. This dynamic and reversible mRNA recruitment to active synapses would allow translation and synaptic remodeling in a spatially and temporally adaptive manner.

Suggested Citation

  • Karl E. Bauer & Inmaculada Segura & Imre Gaspar & Volker Scheuss & Christin Illig & Georg Ammer & Saskia Hutten & Eugénia Basyuk & Sandra M. Fernández-Moya & Janina Ehses & Edouard Bertrand & Michael , 2019. "Live cell imaging reveals 3′-UTR dependent mRNA sorting to synapses," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11123-x
    DOI: 10.1038/s41467-019-11123-x
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    Cited by:

    1. Lance T. Denes & Chase P. Kelley & Eric T. Wang, 2021. "Microtubule-based transport is essential to distribute RNA and nascent protein in skeletal muscle," Nature Communications, Nature, vol. 12(1), pages 1-19, December.

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