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Dissecting a heterotic gene through GradedPool-Seq mapping informs a rice-improvement strategy

Author

Listed:
  • Changsheng Wang

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Shican Tang

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Qilin Zhan

    (Chinese Academy of Sciences)

  • Qingqing Hou

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Yan Zhao

    (Chinese Academy of Sciences)

  • Qiang Zhao

    (Chinese Academy of Sciences)

  • Qi Feng

    (Chinese Academy of Sciences)

  • Congcong Zhou

    (Chinese Academy of Sciences)

  • Danfeng Lyu

    (Chinese Academy of Sciences)

  • Lingling Cui

    (Chinese Academy of Sciences)

  • Yan Li

    (Chinese Academy of Sciences)

  • Jiashun Miao

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Chuanrang Zhu

    (Chinese Academy of Sciences)

  • Yiqi Lu

    (Chinese Academy of Sciences)

  • Yongchun Wang

    (Chinese Academy of Sciences)

  • Ziqun Wang

    (Chinese Academy of Sciences)

  • Jingjie Zhu

    (Chinese Academy of Sciences)

  • Yingying Shangguan

    (Chinese Academy of Sciences)

  • Junyi Gong

    (Chinese Academy of Agricultural Sciences)

  • Shihua Yang

    (Chinese Academy of Agricultural Sciences)

  • Wuqi Wang

    (Fujian Academy of Agricultural Sciences)

  • Jianfu Zhang

    (Fujian Academy of Agricultural Sciences)

  • Huaan Xie

    (Fujian Academy of Agricultural Sciences)

  • Xuehui Huang

    (Shanghai Normal University)

  • Bin Han

    (Chinese Academy of Sciences)

Abstract

Hybrid rice breeding for exploiting hybrid vigor, heterosis, has greatly increased grain yield. However, the heterosis-related genes associated with rice grain production remain largely unknown, partly because comprehensive mapping of heterosis-related traits is still labor-intensive and time-consuming. Here, we present a quantitative trait locus (QTL) mapping method, GradedPool-Seq, for rapidly mapping QTLs by whole-genome sequencing of graded-pool samples from F2 progeny via bulked-segregant analysis. We implement this method and map-based cloning to dissect the heterotic QTL GW3p6 from the female line. We then generate the near isogenic line NIL-FH676::GW3p6 by introgressing the GW3p6 allele from the female line Guangzhan63-4S into the male inbred line Fuhui676. The NIL-FH676::GW3p6 exhibits grain yield highly increased compared to Fuhui676. This study demonstrates that it may be possible to achieve a high level of grain production in inbred rice lines without the need to construct hybrids.

Suggested Citation

  • Changsheng Wang & Shican Tang & Qilin Zhan & Qingqing Hou & Yan Zhao & Qiang Zhao & Qi Feng & Congcong Zhou & Danfeng Lyu & Lingling Cui & Yan Li & Jiashun Miao & Chuanrang Zhu & Yiqi Lu & Yongchun Wa, 2019. "Dissecting a heterotic gene through GradedPool-Seq mapping informs a rice-improvement strategy," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11017-y
    DOI: 10.1038/s41467-019-11017-y
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