IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-10891-w.html
   My bibliography  Save this article

Tethering of vesicles to the Golgi by GMAP210 controls LAT delivery to the immune synapse

Author

Listed:
  • Andres Ernesto Zucchetti

    (Integrative analysis of T cell activation team)

  • Laurence Bataille

    (Integrative analysis of T cell activation team)

  • Jean-Marie Carpier

    (Integrative analysis of T cell activation team
    Yale University School of Medicine)

  • Stéphanie Dogniaux

    (Integrative analysis of T cell activation team)

  • Mabel San Roman-Jouve

    (Integrative analysis of T cell activation team)

  • Mathieu Maurin

    (Integrative analysis of T cell activation team)

  • Michael W. Stuck

    (University of Massachusetts Medical School)

  • Rosa M. Rios

    (CABIMER-CSIC/US/UPO)

  • Cosima T. Baldari

    (University of Siena)

  • Gregory J. Pazour

    (University of Massachusetts Medical School)

  • Claire Hivroz

    (Integrative analysis of T cell activation team)

Abstract

The T cell immune synapse is a site of intense vesicular trafficking. Here we show that the golgin GMAP210, known to capture vesicles and organize membrane traffic at the Golgi, is involved in the vesicular transport of LAT to the immune synapse. Upon activation, more GMAP210 interact with LAT-containing vesicles and go together with LAT to the immune synapse. Regulating LAT recruitment and LAT-dependent signaling, GMAP210 controls T cell activation. Using a rerouting and capture assay, we show that GMAP210 captures VAMP7-decorated vesicles. Overexpressing different domains of GMAP210, we also show that GMAP210 allows their specific delivery to the immune synapse by tethering LAT-vesicles to the Golgi. Finally, in a model of ectopic expression of LAT in ciliated cells, we show that GMAP210 tethering activity controls the delivery of LAT to the cilium. Hence, our results reveal a function for the golgin GMAP210 conveying specific vesicles to the immune synapse.

Suggested Citation

  • Andres Ernesto Zucchetti & Laurence Bataille & Jean-Marie Carpier & Stéphanie Dogniaux & Mabel San Roman-Jouve & Mathieu Maurin & Michael W. Stuck & Rosa M. Rios & Cosima T. Baldari & Gregory J. Pazou, 2019. "Tethering of vesicles to the Golgi by GMAP210 controls LAT delivery to the immune synapse," Nature Communications, Nature, vol. 10(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10891-w
    DOI: 10.1038/s41467-019-10891-w
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-10891-w
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-10891-w?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10891-w. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.