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Oxidative post-translational modification of EXECUTER1 is required for singlet oxygen sensing in plastids

Author

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  • Vivek Dogra

    (Chinese Academy of Sciences)

  • Mingyue Li

    (Chinese Academy of Sciences
    University of the Chinese Academy of Sciences)

  • Somesh Singh

    (Chinese Academy of Sciences)

  • Mengping Li

    (Chinese Academy of Sciences
    University of the Chinese Academy of Sciences)

  • Chanhong Kim

    (Chinese Academy of Sciences
    University of the Chinese Academy of Sciences)

Abstract

Environmental information perceived by chloroplasts can be translated into retrograde signals that alter the expression of nuclear genes. Singlet oxygen (1O2) generated by photosystem II (PSII) can cause photo-oxidative damage of PSII but has also been implicated in retrograde signaling. We previously reported that a nuclear-encoded chloroplast FtsH2 metalloprotease coordinates 1O2-triggered retrograde signaling by promoting the degradation of the EXECUTER1 (EX1) protein, a putative 1O2 sensor. Here, we show that a 1O2-mediated oxidative post-translational modification of EX1 is essential for initiating 1O2-derived signaling. Specifically, the Trp643 residue in DUF3506 domain of EX1 is prone to oxidation by 1O2. Both the substitution of Trp643 with 1O2-insensitive amino acids and the deletion of the DUF3506 domain abolish the EX1-mediated 1O2 signaling. We thus provide mechanistic insight into how EX1 senses 1O2 via Trp643 located in the DUF3506 domain.

Suggested Citation

  • Vivek Dogra & Mingyue Li & Somesh Singh & Mengping Li & Chanhong Kim, 2019. "Oxidative post-translational modification of EXECUTER1 is required for singlet oxygen sensing in plastids," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10760-6
    DOI: 10.1038/s41467-019-10760-6
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