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Coherent diffractive imaging of microtubules using an X-ray laser

Author

Listed:
  • Gisela Brändén

    (University of Gothenburg)

  • Greger Hammarin

    (University of Gothenburg)

  • Rajiv Harimoorthy

    (University of Gothenburg)

  • Alexander Johansson

    (University of Gothenburg)

  • David Arnlund

    (University of Gothenburg)

  • Erik Malmerberg

    (Lawrence Berkeley National Laboratory)

  • Anton Barty

    (Deutsches Elektronen-Synchrotron DESY)

  • Stefan Tångefjord

    (University of Gothenburg)

  • Peter Berntsen

    (University of Gothenburg)

  • Daniel P. DePonte

    (SLAC National Accelerator Laboratory)

  • Carolin Seuring

    (Deutsches Elektronen-Synchrotron DESY
    The Hamburg Center for Ultrafast Imaging)

  • Thomas A. White

    (Deutsches Elektronen-Synchrotron DESY)

  • Francesco Stellato

    (Deutsches Elektronen-Synchrotron DESY)

  • Richard Bean

    (Deutsches Elektronen-Synchrotron DESY)

  • Kenneth R. Beyerlein

    (Deutsches Elektronen-Synchrotron DESY)

  • Leonard M. G. Chavas

    (Deutsches Elektronen-Synchrotron DESY)

  • Holger Fleckenstein

    (Deutsches Elektronen-Synchrotron DESY)

  • Cornelius Gati

    (Deutsches Elektronen-Synchrotron DESY)

  • Umesh Ghoshdastider

    (Biopolis, A*STAR (Agency for Science, Technology and Research))

  • Lars Gumprecht

    (Deutsches Elektronen-Synchrotron DESY)

  • Dominik Oberthür

    (Deutsches Elektronen-Synchrotron DESY)

  • David Popp

    (Biopolis, A*STAR (Agency for Science, Technology and Research))

  • Marvin Seibert

    (SLAC National Accelerator Laboratory)

  • Thomas Tilp

    (Deutsches Elektronen-Synchrotron DESY)

  • Marc Messerschmidt

    (SLAC National Accelerator Laboratory)

  • Garth J. Williams

    (SLAC National Accelerator Laboratory)

  • N. Duane Loh

    (National University of Singapore)

  • Henry N. Chapman

    (Deutsches Elektronen-Synchrotron DESY
    The Hamburg Center for Ultrafast Imaging
    University of Hamburg)

  • Peter Zwart

    (Lawrence Berkeley National Laboratory)

  • Mengning Liang

    (Deutsches Elektronen-Synchrotron DESY
    SLAC National Accelerator Laboratory)

  • Sébastien Boutet

    (SLAC National Accelerator Laboratory)

  • Robert C. Robinson

    (Biopolis, A*STAR (Agency for Science, Technology and Research)
    National University of Singapore
    Okayama University)

  • Richard Neutze

    (University of Gothenburg)

Abstract

X-ray free electron lasers (XFELs) create new possibilities for structural studies of biological objects that extend beyond what is possible with synchrotron radiation. Serial femtosecond crystallography has allowed high-resolution structures to be determined from micro-meter sized crystals, whereas single particle coherent X-ray imaging requires development to extend the resolution beyond a few tens of nanometers. Here we describe an intermediate approach: the XFEL imaging of biological assemblies with helical symmetry. We collected X-ray scattering images from samples of microtubules injected across an XFEL beam using a liquid microjet, sorted these images into class averages, merged these data into a diffraction pattern extending to 2 nm resolution, and reconstructed these data into a projection image of the microtubule. Details such as the 4 nm tubulin monomer became visible in this reconstruction. These results illustrate the potential of single-molecule X-ray imaging of biological assembles with helical symmetry at room temperature.

Suggested Citation

  • Gisela Brändén & Greger Hammarin & Rajiv Harimoorthy & Alexander Johansson & David Arnlund & Erik Malmerberg & Anton Barty & Stefan Tångefjord & Peter Berntsen & Daniel P. DePonte & Carolin Seuring & , 2019. "Coherent diffractive imaging of microtubules using an X-ray laser," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10448-x
    DOI: 10.1038/s41467-019-10448-x
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