IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-10140-0.html
   My bibliography  Save this article

Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila

Author

Listed:
  • Hagar Meltzer

    (Weizmann Institute of Science)

  • Efrat Marom

    (Weizmann Institute of Science)

  • Idan Alyagor

    (Weizmann Institute of Science)

  • Oded Mayseless

    (Weizmann Institute of Science)

  • Victoria Berkun

    (Weizmann Institute of Science)

  • Netta Segal-Gilboa

    (Weizmann Institute of Science)

  • Tamar Unger

    (Weizmann Institute of Science)

  • David Luginbuhl

    (Stanford University)

  • Oren Schuldiner

    (Weizmann Institute of Science)

Abstract

Gene editing by CRISPR/Cas9 is commonly used to generate germline mutations or perform in vitro screens, but applicability for in vivo screening has so far been limited. Recently, it was shown that in Drosophila, Cas9 expression could be limited to a desired group of cells, allowing tissue-specific mutagenesis. Here, we thoroughly characterize tissue-specific (ts)CRISPR within the complex neuronal system of the Drosophila mushroom body. We report the generation of a library of gRNA-expressing plasmids and fly lines using optimized tools, which provides a valuable resource to the fly community. We demonstrate the application of our library in a large-scale in vivo screen, which reveals insights into developmental neuronal remodeling.

Suggested Citation

  • Hagar Meltzer & Efrat Marom & Idan Alyagor & Oded Mayseless & Victoria Berkun & Netta Segal-Gilboa & Tamar Unger & David Luginbuhl & Oren Schuldiner, 2019. "Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10140-0
    DOI: 10.1038/s41467-019-10140-0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-10140-0
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-10140-0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10140-0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.