Author
Listed:
- Bernat Blasco-Moreno
(Universitat Pompeu Fabra)
- Leire Campos-Mata
(Universitat Pompeu Fabra)
- René Böttcher
(Universitat Pompeu Fabra)
- José García-Martínez
(Universitat de València)
- Jennifer Jungfleisch
(Universitat Pompeu Fabra)
- Danny D. Nedialkova
(Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine
Mechanisms of Protein Biogenesis Laboratory, Max Planck Institute of Biochemistry)
- Shiladitya Chattopadhyay
(Technion-Israel Institute of Technology)
- María-Eugenia Gas
(Universitat Pompeu Fabra)
- Baldomero Oliva
(Universitat Pompeu Fabra)
- José E. Pérez-Ortín
(Universitat de València)
- Sebastian A. Leidel
(Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine
University of Bern)
- Mordechai Choder
(Technion-Israel Institute of Technology)
- Juana Díez
(Universitat Pompeu Fabra)
Abstract
The highly conserved 5’–3’ exonuclease Xrn1 regulates gene expression in eukaryotes by coupling nuclear DNA transcription to cytosolic mRNA decay. By integrating transcriptome-wide analyses of translation with biochemical and functional studies, we demonstrate an unanticipated regulatory role of Xrn1 in protein synthesis. Xrn1 promotes translation of a specific group of transcripts encoding membrane proteins. Xrn1-dependence for translation is linked to poor structural RNA contexts for translation initiation, is mediated by interactions with components of the translation initiation machinery and correlates with an Xrn1-dependence for mRNA localization at the endoplasmic reticulum, the translation compartment of membrane proteins. Importantly, for this group of mRNAs, Xrn1 stimulates transcription, mRNA translation and decay. Our results uncover a crosstalk between the three major stages of gene expression coordinated by Xrn1 to maintain appropriate levels of membrane proteins.
Suggested Citation
Bernat Blasco-Moreno & Leire Campos-Mata & René Böttcher & José García-Martínez & Jennifer Jungfleisch & Danny D. Nedialkova & Shiladitya Chattopadhyay & María-Eugenia Gas & Baldomero Oliva & José E. , 2019.
"The exonuclease Xrn1 activates transcription and translation of mRNAs encoding membrane proteins,"
Nature Communications, Nature, vol. 10(1), pages 1-15, December.
Handle:
RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09199-6
DOI: 10.1038/s41467-019-09199-6
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