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Nuclear lamina integrity is required for proper spatial organization of chromatin in Drosophila

Author

Listed:
  • Sergey V. Ulianov

    (Russian Academy of Sciences
    M.V. Lomonosov Moscow State University)

  • Semen A. Doronin

    (Russian Academy of Sciences)

  • Ekaterina E. Khrameeva

    (Skolkovo Institute of Science and Technology
    Russian Academy of Sciences)

  • Pavel I. Kos

    (M.V. Lomonosov Moscow State University)

  • Artem V. Luzhin

    (Russian Academy of Sciences)

  • Sergei S. Starikov

    (M.V. Lomonosov Moscow State University)

  • Aleksandra A. Galitsyna

    (Russian Academy of Sciences
    Skolkovo Institute of Science and Technology
    Russian Academy of Sciences
    M.V. Lomonosov Moscow State University)

  • Valentina V. Nenasheva

    (Russian Academy of Sciences)

  • Artem A. Ilyin

    (Russian Academy of Sciences)

  • Ilya M. Flyamer

    (University of Edinburgh)

  • Elena A. Mikhaleva

    (Russian Academy of Sciences)

  • Mariya D. Logacheva

    (Skolkovo Institute of Science and Technology
    M.V. Lomonosov Moscow State University
    Kazan Federal University)

  • Mikhail S. Gelfand

    (Skolkovo Institute of Science and Technology
    Russian Academy of Sciences
    National Research University Higher School of Economics)

  • Alexander V. Chertovich

    (M.V. Lomonosov Moscow State University)

  • Alexey A. Gavrilov

    (Russian Academy of Sciences)

  • Sergey V. Razin

    (Russian Academy of Sciences
    M.V. Lomonosov Moscow State University)

  • Yuri Y. Shevelyov

    (Russian Academy of Sciences)

Abstract

How the nuclear lamina (NL) impacts on global chromatin architecture is poorly understood. Here, we show that NL disruption in Drosophila S2 cells leads to chromatin compaction and repositioning from the nuclear envelope. This increases the chromatin density in a fraction of topologically-associating domains (TADs) enriched in active chromatin and enhances interactions between active and inactive chromatin. Importantly, upon NL disruption the NL-associated TADs become more acetylated at histone H3 and less compact, while background transcription is derepressed. Two-colour FISH confirms that a TAD becomes less compact following its release from the NL. Finally, polymer simulations show that chromatin binding to the NL can per se compact attached TADs. Collectively, our findings demonstrate a dual function of the NL in shaping the 3D genome. Attachment of TADs to the NL makes them more condensed but decreases the overall chromatin density in the nucleus by stretching interphase chromosomes.

Suggested Citation

  • Sergey V. Ulianov & Semen A. Doronin & Ekaterina E. Khrameeva & Pavel I. Kos & Artem V. Luzhin & Sergei S. Starikov & Aleksandra A. Galitsyna & Valentina V. Nenasheva & Artem A. Ilyin & Ilya M. Flyame, 2019. "Nuclear lamina integrity is required for proper spatial organization of chromatin in Drosophila," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09185-y
    DOI: 10.1038/s41467-019-09185-y
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