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The Wolbachia mobilome in Culex pipiens includes a putative plasmid

Author

Listed:
  • Julie Reveillaud

    (University of Montpellier)

  • Sarah R. Bordenstein

    (Vanderbilt University)

  • Corinne Cruaud

    (Commissariat à l’Energie Atomique et aux Energies Alternatives (CEA), Institut de Biologie François Jacob, Genoscope)

  • Alon Shaiber

    (University of Chicago
    University of Chicago)

  • Özcan C. Esen

    (University of Chicago)

  • Mylène Weill

    (UMR CNRS-IRD-EPHE-Université de Montpellier)

  • Patrick Makoundou

    (UMR CNRS-IRD-EPHE-Université de Montpellier)

  • Karen Lolans

    (University of Chicago)

  • Andrea R. Watson

    (University of Chicago)

  • Ignace Rakotoarivony

    (University of Montpellier)

  • Seth R. Bordenstein

    (Vanderbilt University
    Vanderbilt University
    Vanderbilt University)

  • A. Murat Eren

    (University of Chicago
    University of Chicago
    Marine Biological Laboratory)

Abstract

Wolbachia is a genus of obligate intracellular bacteria found in nematodes and arthropods worldwide, including insect vectors that transmit dengue, West Nile, and Zika viruses. Wolbachia’s unique ability to alter host reproductive behavior through its temperate bacteriophage WO has enabled the development of new vector control strategies. However, our understanding of Wolbachia’s mobilome beyond its bacteriophages is incomplete. Here, we reconstruct near-complete Wolbachia genomes from individual ovary metagenomes of four wild Culex pipiens mosquitoes captured in France. In addition to viral genes missing from the Wolbachia reference genome, we identify a putative plasmid (pWCP), consisting of a 9.23-kbp circular element with 14 genes. We validate its presence in additional Culex pipiens mosquitoes using PCR, long-read sequencing, and screening of existing metagenomes. The discovery of this previously unrecognized extrachromosomal element opens additional possibilities for genetic manipulation of Wolbachia.

Suggested Citation

  • Julie Reveillaud & Sarah R. Bordenstein & Corinne Cruaud & Alon Shaiber & Özcan C. Esen & Mylène Weill & Patrick Makoundou & Karen Lolans & Andrea R. Watson & Ignace Rakotoarivony & Seth R. Bordenstei, 2019. "The Wolbachia mobilome in Culex pipiens includes a putative plasmid," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08973-w
    DOI: 10.1038/s41467-019-08973-w
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