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Morphologic determinant of tight junctions revealed by claudin-3 structures

Author

Listed:
  • Shun Nakamura

    (Nagoya University
    Nagoya University)

  • Katsumasa Irie

    (Nagoya University
    Nagoya University)

  • Hiroo Tanaka

    (Osaka University)

  • Kouki Nishikawa

    (Nagoya University)

  • Hiroshi Suzuki

    (Nagoya University
    The Rockefeller University)

  • Yasunori Saitoh

    (Nagoya University
    Okayama University)

  • Atsushi Tamura

    (Osaka University)

  • Sachiko Tsukita

    (Osaka University)

  • Yoshinori Fujiyoshi

    (Nagoya University
    CeSPIA Inc.)

Abstract

Tight junction is a cell adhesion apparatus functioning as barrier and/or channel in the paracellular spaces of epithelia. Claudin is the major component of tight junction and polymerizes to form tight junction strands with various morphologies that may correlate with their functions. Here we present the crystal structure of mammalian claudin-3 at 3.6 Å resolution. The third transmembrane helix of claudin-3 is clearly bent compared with that of other subtypes. Structural analysis of additional two mutants with a single mutation representing other subtypes in the third helix indicates that this helix takes a bent or straight structure depending on the residue. The presence or absence of the helix bending changes the positions of residues related to claudin-claudin interactions and affects the morphology and adhesiveness of the tight junction strands. These results evoke a model for tight junction strand formation with different morphologies – straight or curvy strands – observed in native epithelia.

Suggested Citation

  • Shun Nakamura & Katsumasa Irie & Hiroo Tanaka & Kouki Nishikawa & Hiroshi Suzuki & Yasunori Saitoh & Atsushi Tamura & Sachiko Tsukita & Yoshinori Fujiyoshi, 2019. "Morphologic determinant of tight junctions revealed by claudin-3 structures," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08760-7
    DOI: 10.1038/s41467-019-08760-7
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    Cited by:

    1. Hannes Gonschior & Christopher Schmied & Rozemarijn Eva Van der Veen & Jenny Eichhorst & Nina Himmerkus & Jörg Piontek & Dorothee Günzel & Markus Bleich & Mikio Furuse & Volker Haucke & Martin Lehmann, 2022. "Nanoscale segregation of channel and barrier claudins enables paracellular ion flux," Nature Communications, Nature, vol. 13(1), pages 1-20, December.

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