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Plasmodium vivax transcriptomes reveal stage-specific chloroquine response and differential regulation of male and female gametocytes

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  • Adam Kim

    (University of Maryland School of Medicine)

  • Jean Popovici

    (Institut Pasteur in Cambodia)

  • Didier Menard

    (Institut Pasteur in Cambodia
    Institut Pasteur)

  • David Serre

    (University of Maryland School of Medicine)

Abstract

Studies of Plasmodium vivax gene expression are complicated by the lack of in vitro culture system and the difficulties associated with studying clinical infections that often contain multiple clones and a mixture of parasite stages. Here, we characterize the transcriptomes of P. vivax parasites from 26 malaria patients. We show that most parasite mRNAs derive from trophozoites and that the asynchronicity of P. vivax infections is therefore unlikely to confound gene expression studies. Analyses of gametocyte genes reveal two distinct clusters of co-regulated genes, suggesting that male and female gametocytes are independently regulated. Finally, we analyze gene expression changes induced by chloroquine and show that this antimalarial drug efficiently eliminates most P. vivax parasite stages but, in contrast to P. falciparum, does not affect trophozoites.

Suggested Citation

  • Adam Kim & Jean Popovici & Didier Menard & David Serre, 2019. "Plasmodium vivax transcriptomes reveal stage-specific chloroquine response and differential regulation of male and female gametocytes," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08312-z
    DOI: 10.1038/s41467-019-08312-z
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