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Screening and Production of Lipase Producing Bacteria from Vegetable Oil Industry

Author

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  • Veerapagu. M
  • Jeya K. R
  • Sankaranarayanan. A

Abstract

Lipases are industrially important enzymes having applications in numerous industries. The present study aims to isolate different bacteria and screen them for lipase production. Oil spilled soil samples were collected from vegetable oil industry of Harur, Dharmapuri district. A total of thirteen morphologically distinct bacteria isolated were screened for lipase production in Tween 80 agar plate. Lipolytic activity was confirmed by zone of precipitation around the colony. Among thirteen isolates, six isolates exhibited lipolytic activity in plate assay. The bacterial isolate HLB7 produced high amount of lipase 2.6U/ml in lipase production medium by submerged fermentation was identified as Pseudomonas sp. The optimum incubation time, inoculum size, temperature and pH for lipase production were determined. The lipase production was maximum at incubation time 48 hr (3.26U/ml), Inoculum size 4% (4.37U/ml), temperature 40?C (4.65U/ml) and pH 7 ( 4.95U/ml) by the Pseudomonas sp

Suggested Citation

  • Veerapagu. M & Jeya K. R & Sankaranarayanan. A, 2016. "Screening and Production of Lipase Producing Bacteria from Vegetable Oil Industry," International Journal of Scientific Research in Science, Engineering and Technology, International Journal of Scientific Research in Science, Engineering and Technology, vol. 2(6), pages 726-732, December.
    • Handle: RePEc:ijs:ijsrse:v2:y2016:i6:id:hijsrset1626172
    Note: Article URL: https://ijsrset.com/IJSRSET1626172
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