Environmental and Microbiological Performance of a CAM-Compliant Green Cleaning Protocol: An Integrated Life Cycle and Surface Contamination Assessment in a Civil Facility

The transition toward sustainable facility management requires cleaning systems that reduce environmental burdens while maintaining high hygienic standards. This study presents a comparative evaluation of a green cleaning protocol (EVA SmartClean), compliant with the Italian Minimum Environmental Criteria (CAM; D.M. 29 January 2021), compared with a conventional cleaning system implemented in a civil facility (Adriatico Guest House, Trieste, Italy; 8260 m 2 ). The assessment integrates a cradle-to-grave Life Cycle Assessment (LCA), conducted in accordance with ISO 14040, ISO 14044, ISO 14067 and PCR 2011:03 for professional cleaning services, with an extensive microbiological surface monitoring campaign performed using RODAC plates and swab sampling. The functional unit was defined as 1 m 2 of representative surface maintained clean for one year. The green protocol achieved a 47.7% reduction in Global Warming Potential (GWP100 based on IPCC AR6 characterization factors), corresponding to −110 g CO 2 e/m 2 ·year and −908 kg CO 2 e/year for the entire facility. Major reductions in climate impact were associated with chemical consumption (−82.6%), energy use (−49.5%), and textile waste generation (−92.4%). Microbiological analyses demonstrated that both protocols complied with reference hygiene thresholds, while the green system achieved reductions in total mesophilic counts that were comparable or superior across representative surfaces. The results confirm that environmental optimization in cleaning services can be achieved without compromising microbiological safety, supporting public procurement policies aligned with CAM requirements and Sustainable Development Goals (SDGs 12 and 13).