A Resource-Efficient Morpho-Statistical Protocol (AMSP) for Functional Cave Zonation: Enhancing Sustainable Management of Subterranean Heritage

Caves are fragile subterranean ecosystems whose conservation depends on accurate microclimatic zonation. Traditional fixed-distance sampling often overlooks non-linear thermodynamic transitions at geomorphological thresholds, hindering sustainable management of subterranean biodiversity. This study introduces the Adaptive Morpho-Statistical Protocol (AMSP), a novel, resource-efficient framework for functional cave profiling. The methodology integrates high-precision atmospheric monitoring with adaptive spatial positioning to identify three distinct sectors (S1–S3) based on thermodynamic homeostasis rather than linear distance. Validated across five diverse cave archetypes in the Vratsa Karst Region (Bulgaria), the AMSP demonstrated exceptional predictive power using second-order polynomial regressions (R 2 > 0.92). A key finding is the definition of a standardized reference threshold for deep-reach stability (Sector 3), consistently characterized by a Dew Point Standard Deviation (SD DP < 0.40) and stabilized thermal coupling (∆T → 0). Furthermore, the adaptive strategy successfully captured extreme hygrometric jumps at morphological bottlenecks—critical inflection points for protecting sensitive biota. By providing a cost-effective and replicable standard, the AMSP bridges the gap between spatial resolution and logistical feasibility in challenging environments. These results confirm that morphological isolation is the primary driver of microclimatic inertia, offering a robust tool for sustainable subterranean heritage management and high-precision ecological monitoring in protected karst landscapes.