RSM- and ANN-Based Optimization and Modeling of Pollutant Reduction and Biomass Production of Azolla pinnata Using Paper Mill Effluent

The discharge of untreated paper mill effluent poses significant ecological risks due to its high organic and nutrient loads. This study aimed to assess the phytoremediation potential of Azolla pinnata for treating paper mill effluent. Response Surface Methodology (RSM) and Artificial Neural Network (ANN) modeling approaches were applied and optimization was used for pollutant removal and plant biomass production. Experiments were designed using a Central Composite Design with two independent variables: effluent concentration (0, 50, and 100%) and plant density (10, 20, and 30 g per container). The responses measured were biochemical oxygen demand (BOD), chemical oxygen demand (COD) removal efficiencies, and final biomass yield after 16 days of exposure. RSM produced statistically significant ( p < 0.05) second-order regression models for all three responses (coefficient of determination; R 2 > 0.98), while ANN showed slightly lower prediction errors within the experimental range studied. Maximum observed removal efficiencies were 91.74% for BOD, 80.91% for COD, and 92.66 g biomass yield under 50% effluent concentration and 30 g plant density. Optimization via both models suggested closely comparable operating conditions (79% effluent concentration and 29 g biomass) for optimal performance. The results indicate that A. pinnata demonstrates potential as a low-cost, nature-based treatment system for industrial effluent remediation under controlled conditions. The integration of data-driven optimization with biological treatment contributes to sustainable effluent management strategies by reducing chemical inputs, minimizing energy demand, and enabling biomass generation with potential downstream valorization.