Effect of Intercropping Paulownia with Spring Barley on Biodiversity in Agroecosystems Under Polish Conditions

The study evaluated the effect of intercropping Paulownia ( Paulownia spp.) with spring barley ( Hordeum vulgare L., cv. KWS Thalis) on selected components of agroecosystem biodiversity under Polish conditions. A field experiment established in 2019 compared an alley cropping system with barley monoculture during the 2025 growing season. Weed infestation, soil microbial communities, mesofauna abundance, and crop yield were assessed. Weed abundance was lower in the intercropping system than in monoculture, reaching 5.6 vs. 15.6 plants m −2 at BBCH 21 and 21 and 22.8 vs. 35.6 plants m −2 at BBCH 75. Bacterial alpha diversity was significantly higher under intercropping conditions, with Shannon index values ranging from 5.12 to 5.25, compared with 4.98–5.09 in monoculture. Fungal diversity showed moderate differences between systems, whereas the abundance of Collembola and Acari was influenced mainly by seasonal variation rather than by cultivation system. No significant reduction in barley yield was observed under intercropping conditions. The results suggest that Paulownia-based alley cropping may reduce weed pressure and support selected soil biological properties without negatively affecting crop productivity. However, the observed responses varied depending on the analyzed parameter and sampling period, indicating the preliminary and context-dependent character of the results. Further long-term studies are required to better understand the ecological mechanisms operating in such agroforestry systems.