Environmental Microbiological Sampling in Civil Settings: Comparative LCA Analysis of Green Cleaning Techniques vs. Traditional Methods in Accordance with New Italian CAM Guidelines

This research conducts a comparative life cycle assessment (LCA) to examine both the ecological footprint and microbiological performance of eco-friendly and traditional cleaning methods in non-clinical environments. Conducted in accordance with the updated Minimum Environmental Criteria (CAMs), the research follows the principles and framework established by UNI EN ISO 14040 and 14044. Additionally, the assessment of carbon footprint (kg CO 2 e) adheres to ISO 14067:2018, with guidance from Product Category Rules (PCR 2011:03 v3.0.1). Our findings demonstrate that the Green Protocol implemented by Dussmann Service reduces the carbon footprint of cleaning activities by 20.0% compared to the traditional protocol, equating to an annual reduction of 156 kg CO 2 at the pilot site. Laboratory analyses confirm that the Green Protocol maintains hygiene standards equivalent to conventional methods, ensuring adequate microbiological quality while significantly lowering environmental impact. The study highlights the feasibility of integrating eco-friendly cleaning practices without compromising effectiveness. Future research should explore the scalability, cost-efficiency, and long-term benefits of this approach. This assessment provides a scientifically validated foundation for adopting sustainable cleaning methodologies in professional settings, supporting the transition towards environmentally responsible facility management.