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New Biotechnological Production of EPA by Pythium irregulare Using Alternative Sustainable Media Obtained from Food Industry By-Products and Waste

Author

Listed:
  • Giovanni Luca Russo

    (Centre for Food Innovation and Development in the Food Industry, University of Naples Federico II, 80055 Portici, Italy)

  • Antonio Luca Langellotti

    (Centre for Food Innovation and Development in the Food Industry, University of Naples Federico II, 80055 Portici, Italy)

  • Beatriz Martín-García

    (Department of Nutrition and Food Science, Campus of Cartuja, University of Granada, 18071 Granada, Spain
    Institute of Nutrition and Food Technology ‘José Mataix’, Biomedical Research Center, University of Granada, Avda del Conocimiento sn., 18100 Granada, Spain)

  • Vito Verardo

    (Department of Nutrition and Food Science, Campus of Cartuja, University of Granada, 18071 Granada, Spain
    Institute of Nutrition and Food Technology ‘José Mataix’, Biomedical Research Center, University of Granada, Avda del Conocimiento sn., 18100 Granada, Spain)

  • Raffaele Romano

    (Unit of Food Science and Technology, Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy)

  • Raffaele Sacchi

    (Unit of Food Science and Technology, Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy)

  • Paolo Masi

    (Centre for Food Innovation and Development in the Food Industry, University of Naples Federico II, 80055 Portici, Italy
    Unit of Food Science and Technology, Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy)

Abstract

Long-chain polyunsaturated fatty acids (LC-PUFAs) have multiple beneficial effects on human health, in particular docosahexaenoic acid (DHA, 22:6, n-3) and eicosapentaenoic acid (EPA, 20:5, n-3). A variety of microorganisms has been used for the commercial production of non-animal-source LC-PUFAs. The oomycetes of the Pythium family are promising EPA producers, and in this work, the optimization of Pythium irregulare growth using food industry by-products and wastes as cheap sources of nutrients was carried out. Sugar cane molasses (SCM), spent brewery yeast (SBY), cheese whey (CW), and expired orange juice (EFJ) were tested. A combination of SBY as a source of nitrogen and EFJ as a source of organic carbon resulted in the best outcome among the other sustainable media ingredients. The optimization of the new medium was conducted through a response surface methodology using EFJ and SBY as factors. The results show a significant positive impact of these factors on biomass productivity ( p < 0.005), with an optimized biomass yield of 14.22 g L −1 , a lipid yield of 2.23 g L −1 , and an EPA concentration of 155 mg L −1 .

Suggested Citation

  • Giovanni Luca Russo & Antonio Luca Langellotti & Beatriz Martín-García & Vito Verardo & Raffaele Romano & Raffaele Sacchi & Paolo Masi, 2023. "New Biotechnological Production of EPA by Pythium irregulare Using Alternative Sustainable Media Obtained from Food Industry By-Products and Waste," Sustainability, MDPI, vol. 15(2), pages 1-15, January.
    • Handle: RePEc:gam:jsusta:v:15:y:2023:i:2:p:1147-:d:1028262
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